Cutting edge: Rapid in vivo killing by memory CD8 T cells
Cutting edge: Rapid in vivo killing by memory CD8 T cells
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DOI：
10.4049/jimmunol.171.1.27
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发表时间：
2003-07-01
影响因子：
4.4
通讯作者：
Ahmed, R
中科院分区：
文献类型：
--
作者：
Barber, DL;Wherry, EJ;Ahmed, R
在这项研究中，我们研究了体内效应和记忆CD 8 T细胞的细胞毒活性。在急性淋巴细胞性脉络丛脑膜炎病毒感染后的CTL应答的峰值，效应CD 8 T细胞表现出非常快速的杀伤，并通过穿孔素依赖性机制在15分钟内开始消除过继转移的靶细胞。尽管通过体外Cr-51释放测定，静息记忆CD 8 T细胞的细胞溶解性差，但在转移到免疫小鼠中后，靶细胞迅速消除（1-4小时内），并且CD 62 L（高）和CD 62 L（低）记忆CD 8 T细胞都能够在体内迅速杀死。引人注目的是，当在每个细胞的基础上直接比较时，记忆CD 8 T细胞在消除靶细胞方面仅比效应细胞稍慢。这些数据表明，病毒特异性记忆性CD 8 T细胞在再次暴露于Ag时可以快速获得细胞毒性功能，并且是比先前认识到的更有效的体内杀伤细胞。
In this study, we examined the cytotoxic activity of effector and memory CD8 T cells in vivo. At the peak of the CTL response following an acute lymphocytic choriomeningitis virus infection, effector CD8 T cells exhibited extremely rapid killing and started to eliminate adoptively transferred target cells within 15 min by a perforin-dependent mechanism. Although resting memory CD8 T cells are poorly cytolytic by in vitro Cr-51 release assays, there was rapid elimination (within 1-4 h) of target cells after transfer into immune mice, and both CD62L(high) and CD62L(low) memory CD8 T cells were able to kill rapidly in vivo. Strikingly, when directly compared on a per cell basis, memory CD8 T cells were only slightly slower than effector cells in eliminating target cells. These data indicate that virus specific memory CD8 T cells can rapidly acquire cytotoxic function upon re-exposure to Ag and are much efficient killers in vivo than previously appreciated.