Identification of Cytotoxic Flavor Chemicals in Top-Selling Electronic Cigarette Refill Fluids
Identification of Cytotoxic Flavor Chemicals in Top-Selling Electronic Cigarette Refill Fluids
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DOI：
10.1038/s41598-019-38978-w
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发表时间：
2019-02-26
影响因子：
4.6
通讯作者：
Talbot, Prue
中科院分区：
文献类型：
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作者：
Hua, My;Omaiye, Esther E.;Talbot, Prue
我们使用互联网调查以及本地和在线销售信息确定了最受欢迎的电子烟（EC）再填充液，量化了其风味化学品，并评估了液体和风味化学品的细胞毒性。“浆果/水果/柑橘”是最受欢迎的EC补充液风味类别。从当地商店购买了20种流行的EC填充液，并通过气相色谱-质谱法对成分风味化学品进行了鉴定和定量。总风味化学品浓度范围为0.6至27.9 mg/ml，在95%的液体中，总风味浓度大于尼古丁浓度。20种最受欢迎的填充液含有99种可量化的风味化学物质;每种填充液含有22至47种风味化学物质，大多数是酯类。一些化学品经常被发现，其中几种存在于大多数产品中。在1%浓度下，80%的再填充液在MTT试验中具有细胞毒性。在MTT试验中，在两种最具细胞毒性的填充液中以最高浓度发现的六种纯标准品的风味化学品是有效的，并且在超过50%的产品中的乙基麦芽酚是最具细胞毒性的。这些数据表明，一些流行的补充液的细胞毒性可归因于其高浓度的风味化学品。
We identified the most popular electronic cigarette (EC) refill fluids using an Internet survey and local and online sales information, quantified their flavor chemicals, and evaluated cytotoxicities of the fluids and flavor chemicals. "Berries/Fruits/Citrus" was the most popular EC refill fluid flavor category. Twenty popular EC refill fluids were purchased from local shops, and the ingredient flavor chemicals were identified and quantified by gas chromatography-mass spectrometry. Total flavor chemical concentrations ranged from 0.6 to 27.9 mg/ml, and in 95% of the fluids, total flavor concentration was greater than nicotine concentration. The 20 most popular refill fluids contained 99 quantifiable flavor chemicals; each refill fluid contained 22 to 47 flavor chemicals, most being esters. Some chemicals were found frequently, and several were present in most products. At a 1% concentration, 80% of the refill fluids were cytotoxic in the MTT assay. Six pure standards of the flavor chemicals found at the highest concentrations in the two most cytotoxic refill fluids were effective in the MTT assay, and ethyl maltol, which was in over 50% of the products, was the most cytotoxic. These data show that the cytotoxicity of some popular refill fluids can be attributed to their high concentrations of flavor chemicals.