Cellular barcoding: A technical appraisal
Cellular barcoding: A technical appraisal
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DOI：
10.1016/j.exphem.2014.05.003
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发表时间：
2014-08-01
影响因子：
2.6
通讯作者：
Perie, Leila
中科院分区：
文献类型：
--
作者：
Naik, Shalin H.;Schumacher, Ton N.;Perie, Leila
细胞条形码涉及用独特的遗传可遗传标识符或条形码标记感兴趣的单个细胞，并且正在成为大规模解决单个细胞命运的强大工具。然而，与许多新技术一样，也出现了各种技术和分析挑战。在这里，我们回顾了这些挑战，并强调了蜂窝条形码的力量和局限性。然后，我们阐述了细胞条形码对理解造血作用的贡献，并概述了该技术的未来潜力。 (C) 2014 年 ISEH - 国际实验血液学会。由爱思唯尔公司出版
Cellular barcoding involves the tagging of individual cells of interest with unique genetic heritable identifiers or barcodes and is emerging as a powerful tool to address individual cell fates on a large scale. However, as with many new technologies, diverse technical and analytical challenges have emerged. Here, we review those challenges and highlight both the power and limitations of cellular barcoding. We then illustrate the contribution of cellular barcoding to the understanding of hematopoiesis and outline the future potential of this technology. (C) 2014 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc.