Singlet Oxygen (1ΔgO2) as the Principal Oxidant in Myeloperoxidase-Mediated Bacterial Killing in Neutrophil Phagosome ☆
Singlet Oxygen (1ΔgO2) as the Principal Oxidant in Myeloperoxidase-Mediated Bacterial Killing in Neutrophil Phagosome ☆
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单线态氧 (1ΔgO2) 作为髓过氧化物酶介导的中性粒细胞吞噬体细菌杀伤的主要氧化剂 ☆
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
M. Nakano
中科院分区：
文献类型：
--
作者：
H. Tatsuzawa;T. Maruyama;K. Hori;Y. Sano;M. Nakano
野生型E.大肠杆菌和番茄红素（一种强大的1 O2猝灭剂）产生的大肠杆菌。以人多形核白细胞为吞噬细胞，研究了大肠杆菌对调理活菌的吞噬作用。而野生型和野生型E.大肠杆菌在吞噬体中的存活率下降很快，但溶胞菌在吞噬体中的存活率比野生型大肠杆菌高1.7倍。大肠杆菌中表达。结果与E.将大肠杆菌菌株暴露于在pH 4.5的髓过氧化物酶-H2 O2-Br −系统（纯1 O2产生系统）中产生的1 O2。HOCl可能在生理条件下在髓过氧化物酶-H2 O2-Cl −系统中产生，但不参与杀菌作用，其原因可以解释为吞噬体中接近中性的pH值，在该pH值下，氯化杀菌作用被广泛减弱。这是第一次在嗜水气单胞菌-细菌吞噬体系统中证实1 O2介导的细菌杀伤作用。
Abstract Intraphagosomal viability of wild type E. coli and lycopene (a powerful 1 O 2 quencher)-producing transformant E. coli was investigated using human polymorphonuclear leukocytes as the cells for phagocytosis of opsonized viable bacteria. While the viability of both wild type and the transformant E. coli decreased very rapidly in the phagosome, but the viability of the lycopene-transformant in phagosomes was about 1.7 times higher than that of wild type E. coli after 5 min of incubation. The results were very similar to the results obtained when E. coli strains were exposed to 1 O 2 generated in myeloperoxidase-H 2 O 2 -Br − system (a pure 1 O 2 generating system) at pH 4.5. The reason for HOCl, which may be generated in the myeloperoxidase-H 2 O 2 -Cl − system under physiological conditions but does not become involved in bactericidal action, could be explained by the near neutral pH in phagosomes at which bacterial killing by chlorination is extensively attenuated. This is the first report which proved 1 O 2 -mediated bacterial killing in neutrophil-bacterial phagosomal system.
DOI：
10.1016/s0021-9258(18)42228-4
发表时间：
1992-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. J. Steinbeck;Ahsan U. Khan;M. J. Karnovsky
通讯作者：
M. J. Steinbeck;Ahsan U. Khan;M. J. Karnovsky
影响因子：
2.9
作者：
BARRETTE, WC;HANNUM, DM;HURST, JK
通讯作者：
HURST, JK