A polyphenol-rich fraction obtained from table grapes decreases adiposity, insulin resistance and markers of inflammation and impacts gut microbiota in high-fat-fed mice.
A polyphenol-rich fraction obtained from table grapes decreases adiposity, insulin resistance and markers of inflammation and impacts gut microbiota in high-fat-fed mice.
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DOI：
10.1016/j.jnutbio.2015.12.021
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
McIntosh M
中科院分区：
文献类型：
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作者：
Collins B;Hoffman J;Martinez K;Grace M;Lila MA;Cockrell C;Nadimpalli A;Chang E;Chuang CC;Zhong W;Mackert J;Shen W;Cooney P;Hopkins R;McIntosh M
本研究的目的是确定食用可提取或不可提取的鲜食葡萄部分是否减少了食用高脂肪美国式饮食的代谢后果。给雄性C57 BL/6 J小鼠喂食低脂肪（LF）饮食、高脂肪（HF）饮食或HF饮食16周，所述HF饮食含有全鲜食葡萄粉（5%w/w）、来自葡萄粉的可提取的富含多酚（HF-EP）级分、不可提取的缺乏多酚（HF-NEP）级分或两种级分的相等组合（HF-EP+NEP）。喂食HF-EP和HF-EP+NEP饮食的小鼠与喂食HF的对照组相比，具有较低的体脂百分比和白色脂肪组织（WAT）量，并且具有改善的葡萄糖耐量。喂食HF-EP+NEP饮食的小鼠与喂食HF的对照组相比具有较低的肝脏重量和甘油三酯（TG）水平。喂食HF-EP+NEP饮食的小鼠与喂食HF的对照组相比，具有较高的激素敏感脂肪酶和脂肪TG脂肪酶的肝脏mRNA水平，并且c反应蛋白的表达降低。在附睾（内脏）WAT中，与喂食HF的对照组相比，喂食HF-EP和HF-EP+NEP饮食的小鼠中几种炎症基因的表达水平较低。与HF-EP和HF-NEP饮食相比，喂食HF饮食的小鼠具有增加的髓过氧化物酶活性和回肠粘膜中紧密连接蛋白闭锁小带-1的受损定位。这些治疗效果中的一些与肠道细菌群落结构的改变有关。总的来说，这些数据表明，来自鲜食葡萄的富含多酚的EP级分减轻了与食用HF饮食相关的许多不良健康后果。
The objective of this study was to determine if consuming an extractable or non-extractable fraction of table grapes reduced the metabolic consequences of consuming a high-fat, American-type diet. Male C57BL/6J mice were fed a low fat (LF) diet, a high fat (HF) diet, or a HF diet containing whole table grape powder (5% w/w), an extractable, polyphenol-rich (HF-EP) fraction, a non-extractable, polyphenol-poor (HF-NEP) fraction, or equal combinations of both fractions (HF-EP+NEP) from grape powder for 16 weeks. Mice fed the HF-EP and HF-EP+NEP diets had lower percentages of body fat and amounts of white adipose tissue (WAT) and improved glucose tolerance compared to the HF-fed controls. Mice fed the HF-EP+NEP diet had lower liver weights and triglyceride (TG) levels compared to the HF-fed controls. Mice fed the HF-EP+NEP diets had higher hepatic mRNA levels of hormone sensitive lipase and adipose TG lipase, and decreased expression of c-reactive protein compared to the HF-fed controls. In epididymal (visceral) WAT, the expression levels of several inflammatory genes were lower in mice fed the HF-EP and HF-EP+NEP diets compared to the HF-fed controls. Mice fed the HF diets had increased myeloperoxidase activity and impaired localization of the tight junction protein zonula occludens-1 in ileal mucosa compared to the HF-EP and HF-NEP diets. Several of these treatment effects were associated with alterations in gut bacterial community structure. Collectively, these data demonstrate that the polyphenol-rich, EP fraction from table grapes attenuated many of the adverse health consequences associated with consuming a HF diet.