Surface Labeling of Enveloped Viruses Assisted by Host Cells
Surface Labeling of Enveloped Viruses Assisted by Host Cells
复制标题
宿主细胞辅助的有包膜病毒的表面标记
DOI：
10.1021/cb2001878
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发表时间：
2012-04-01
影响因子：
4
通讯作者：
Pang, Dai-Wen
中科院分区：
文献类型：
--
作者：
Huang, Bi-Hai;Lin, Yi;Pang, Dai-Wen
病毒的标记为理解病毒本身开辟了新的途径，并促进了病毒在现代生物学，医学和材料中的利用。基于病毒劫持宿主细胞机制以生存和繁殖的特点，提出了一种宿主细胞辅助的策略来标记有包膜病毒。通过简单地用商业1，2-二油酰基-sn-甘油基-3-磷酸乙醇胺-N-（cap生物素基）（钠盐）（Biotin-Cap-PE）饲养Vero细胞，我们获得了其膜系统用生物素修饰的生物素化Vero细胞。随后，伪狂犬病病毒（PrV）在生物素标记的Vero细胞中培养，并在病毒自然组装过程中自发地用Biotin-Cap-PE标记PrV子代。由于采用了病毒的自然组装过程进行标记，避免了基因工程的潜在威胁和难以保持病毒天然生物活性的问题。重要的是，这种包膜病毒的标记策略大大降低了技术复杂性，并允许来自不同背景的研究人员将其应用于他们的特定需求。
Labeling of virus opens new pathways for the understanding of viruses themselves and facilitates the utilization of viruses in modern biology, medicine, and materials. Based on the characteristic that viruses hijack their host cellular machineries to survive and reproduce themselves, a host-cell-assisted strategy is proposed to label enveloped viruses. By simply feeding Vero cells with commercial 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (sodium salt) (Biotin-Cap-PE), we obtained biotinylated Vero cells whose membrane systems were modified with biotin. Subsequently, pseudorabies viruses (PrV) were cultivated in the biotinylated Vero cells, and the PrV progenies were spontaneously labeled with Biotin-Cap-PE during viral natural assembly process. Since the viral natural assembly process was employed for the labeling, potential threats of genetic engineering and difficulties in keeping viral natural bioactivity were avoided. Importantly this labeling strategy for enveloped virus greatly reduced the technical complexity and allows researchers from different backgrounds to apply it for their specified demands.