Bcl-2, Bax, and Bcl-x expression in the CA1 area of the hippocampus following transient forebrain ischemia in the adult gerbil
Bcl-2, Bax, and Bcl-x expression in the CA1 area of the hippocampus following transient forebrain ischemia in the adult gerbil
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DOI：
10.1007/s002210050448
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发表时间：
1998-07-01
影响因子：
2
通讯作者：
Martí, E
中科院分区：
文献类型：
--
作者：
Ferrer, I;López, E;Martí, E
成年沙鼠前脑缺血5分钟后，海马CA 1区出现迟发性神经元死亡。免疫组化和Western blotting检查对照组（年龄匹配，非手术和假手术）和缺血沙土鼠Bcl-2，Bax和Bcl-x。对照组海马CA 1区神经元Bcl-2免疫反应弱，Bax免疫反应强。中度Bcl-x免疫反应观察到控制CAI神经元。Bcl-2和Bcl-x免疫反应在缺血后的CA 1神经元中呈强阳性。Bcl-2、Bax和Bcl-x定位于死亡细胞中，因此表明Bcl-2的表达不足以防止神经细胞死亡。尽管Bcl-x抗体不能区分组织切片中的Bcl-xL和Bcl-xS含量。Western印迹法显示缺血性脑脊髓中对应于Bcl-xS的条带的强度显著增加，但对应于Bcl-xL的条带的强度没有显著增加，从而表明Bcl-xS的增加与成年沙鼠短暂前脑缺血后的延迟细胞死亡有关。
Delayed neuronal death was produced in the CA1 area of the hippocampus following 5 min of forebrain ischemia in adult gerbils. Immunohistochemistry and Western blotting to Bcl-2, Bax, and Bcl-x was examined in control (age-matched, non-operated and sham-operated) and ischemic gerbils. Bcl-2 immunoreactivity was low in CA1 neurons, but Bax was highly expressed in CA1 neurons of control gerbils. Moderate Bcl-x immunoreactivity was observed in control CAI neurons. Strong Bcl-2 and Bcl-x immunoreactivity was found in CA1 neurons following ischemia. Bcl-2, Bax, and Bcl-x were localized in dying cells, thus suggesting that expression of Bcl-2 was not sufficient to prevent nerve cells from dying. Although the Bcl-x antibody does not discriminate between Bcl-xL and Bcl-xS content in tissue sections. Western blots disclosed a marked increase in the intensity of the band corresponding to Bcl-xS, but not of the band corresponding to Bcl-xL in ischemic hippocampi, thus indicating that the increase in Bcl-xS is associated with delayed cell death following transient forebrain ischemia in the adult gerbil.