CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo
CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo
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DOI：
10.1242/dev.00632
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发表时间：
2003-09-01
期刊：
影响因子：
4.6
通讯作者：
Yoder, MC
中科院分区：
文献类型：
--
作者：
Ferkowicz, MJ;Starr, M;Yoder, MC
血小板糖蛋白IIb（α（IIb）; CD 41）构成高度表达的血小板表面整联蛋白的α亚基。我们证明，CD 41作为原始红系祖细胞在胚胎第7天（E7.0）的卵黄囊和高水平的表达，基本上所有的E8.25卵黄囊确定造血祖细胞的最早的标志物。胎儿肝脏和骨髓中的一些永久造血祖细胞也表达CD 41。从骨髓和胎肝细胞中分离的CD 41（dim）和CD 41（lo/-）细胞具有造血干细胞竞争性再生能力，然而，活性在CD 41（lo/-）细胞中富集。CD 41（亮）卵黄囊定形祖细胞共表达CD 61并结合纤维蛋白原，证明了受体功能。因此，CD 41表达标志着小鼠胚胎中原始和永久造血的开始，并作为胎儿肝脏和成人骨髓中一些干细胞和祖细胞群体的标志物持续存在，表明这种整合素的新作用。
The platelet glycoprotein IIb (alpha(IIb); CD41) constitutes the alpha subunit of a highly expressed platelet surface integrin protein. We demonstrate that CD41 serves as the earliest marker of primitive erythroid progenitor cells in the embryonic day 7 (E7.0) yolk sac and high-level expression identifies essentially all E8.25 yolk sac definitive hematopoietic progenitors. Some definitive hematopoietic progenitor cells in the fetal liver and bone marrow also express CD41. Hematopoietic stem cell competitive repopulating ability is present in CD41(dim) and CD41(lo/-) cells isolated from bone marrow and fetal liver cells, however, activity is enriched in the CD41(lo/-) cells. CD41(bright) yolk sac definitive progenitor cells co-express CD61 and bind fibrinogen, demonstrating receptor function. Thus, CD41 expression marks the onset of primitive and definitive hematopoiesis in the murine embryo and persists as a marker of some stem and progenitor cell populations in the fetal liver and adult marrow, suggesting novel roles for this integrin.