Mesenchymal stem cells and platelet-rich plasma enhance bone formation in sinus grafting: a histomorphometric study in minipigs
Mesenchymal stem cells and platelet-rich plasma enhance bone formation in sinus grafting: a histomorphometric study in minipigs
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DOI：
10.1111/j.1600-051x.2008.01220.x
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发表时间：
2008-06-01
影响因子：
6.7
通讯作者：
Marchetti, Claudio
中科院分区：
文献类型：
--
作者：
Pieri, Francesco;Lucarelli, Enrico;Marchetti, Claudio
目的：自体、同种异体和同种异体材料用于鼻窦增强具有特定的缺点，这刺激了对新材料和组织工程结构的持续探索。我们研究了在氟羟基磷灰石（FH）支架上植入间充质干细胞（MSCs）和富血小板血浆（PRP）是否能改善上颌窦移植术中骨的形成和骨与种植体的接触（BIC）。材料和方法：8头小型猪行双侧鼻窦增强术。将MSCs、PRP和FH支架（试验点）或FH单独（对照点）分别移植于每个上颌窦。在截骨的远端，每个鼻窦通过面鼻窦壁放置一颗牙种植体在移植材料中。移植后3个月处死动物，收获植入部位的块切片，准备进行组织形态学分析。结果：12周后，与对照组相比，试验点的骨形成明显增加（42.51%对18.98%;0.001）。再生区试验点的BIC显著高于对照点（23.71%比6.63%,p=0.028）。结论：这些结果表明，与单独使用FH相比，MSCs-PRP联合FH可促进小型猪种植体骨形成和骨整合。
Objectives: Autologous, allogenic, and alloplastic materials for sinus augmentation have specific drawbacks, which has stimulated an ongoing search for new materials and tissue-engineering constructs. We investigated whether mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) seeded on a fluorohydroxyapatite (FH) scaffold can improve bone formation and bone-to-implant contact (BIC) in maxillary sinus grafting.Material and Methods: Bilateral sinus augmentation procedures were performed in eight minipigs. MSCs, PRP, and FH scaffold (test site) or FH alone (control site) were grafted in each maxillary sinus. Distal to the osteotomy, one dental implant per sinus was placed in the grafting material through the facial sinus wall. The animals were killed 3 months after grafting, and block sections of the implant sites were harvested and prepared for histomorphometric analysis.Results: After 12 weeks, a significant increase in bone formation occurred in the test sites compared with the control sites (42.51% versus 18.98%; 0.001). In addition, BIC was significantly greater in the test sites compared with the control sites in the regenerated area (23.71%versus 6.63%; p=0.028).Conclusions: These findings show that sinus augmentation with MSCs-PRP, combined with FH may enhance bone formation and osseointegration of dental implants compared with FH alone in minipigs.