MAL2 selectively regulates polymeric IgA receptor delivery from the Golgi to the plasma membrane in WIF-B cells.
MAL2 selectively regulates polymeric IgA receptor delivery from the Golgi to the plasma membrane in WIF-B cells.
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DOI：
10.1111/j.1600-0854.2010.01074.x
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发表时间：
2010-08
期刊：
影响因子：
--
通讯作者：
Tuma PL
中科院分区：
文献类型：
--
作者：
In JG;Tuma PL
MAL2 has been identified as a hepatic transcytotic regulator that mediates delivery from basolateral endosomes to the sub-apical compartment (SAC). However, overexpression of polymeric immunoglobulin A-receptor (pIgA-R) in polarized, hepatic WIF-B cells led to the dramatic redistribution of MAL2 into the Golgi and all the transcytotic intermediates occupied by the receptor. Although overexpressed hemagglutinin and dipeptidylpeptidase IV (DPPIV) distributed to the same compartments, MAL2 distributions did not change indicating the effect is selective. Cycloheximide treatment led to decreased pIgA-R and MAL2 intracellular staining, first in the Golgi then the SAC, suggesting they were apically delivered and that MAL2 was mediating the process. This was tested in Clone 9 cells (that lack endogenous MAL2). When expressed alone, pIgA-R was restricted to the Golgi whereas when coexpressed with MAL2, it distributed to the surface, was internalized and delivered to MAL2-positive puncta. In contrast, DPPIV distributions were independent of MAL2. Surface delivery of newly synthesized pIgA-R, but not DPPIV, was enhanced >9-fold by MAL2 coexpression. In WIF-B cells where MAL2 expression was knocked down, pIgA-R, but not DPPIV, was retained in the Golgi and its basolateral delivery was impaired. Thus, in addition to its role in transcytosis, MAL2 also regulates pIgA-R delivery from the Golgi to the plasma membrane.
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影响因子：
7.8
作者：
Ihrke, G;Neufeld, E B;Meads, T;Shanks, M R;Cassio, D;Laurent, M;Schroer, T A;Pagano, R E;Hubbard, A L
通讯作者：
Hubbard, A L
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作者：
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通讯作者：
Alonso, MA
影响因子：
4.8
作者：
Martín-Belmonte, F;Arvan, P;Alonso, MA
通讯作者：
Alonso, MA
影响因子：
3.3
作者：
Ramnarayanan, Sai Prasad;Cheng, Christina A.;Tuma, Pamela L.
通讯作者：
Tuma, Pamela L.
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DOI：
10.1083/jcb.141.1.115
发表时间：
1998-04-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ihrke G;Martin GV;Shanks MR;Schrader M;Schroer TA;Hubbard AL
通讯作者：
Hubbard AL