Diapause in the mosquito Culex pipiens evokes a metabolic switch from blood feeding to sugar gluttony
Diapause in the mosquito Culex pipiens evokes a metabolic switch from blood feeding to sugar gluttony
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DOI：
10.1073/pnas.0507958102
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发表时间：
2005-11-01
影响因子：
11.1
通讯作者：
Denlinger, DL
中科院分区：
文献类型：
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作者：
Robich, RM;Denlinger, DL
库蚊越冬休眠（滞育）的一个关键特征是雌性库蚊从嗜血到嗜糖的转变。我们提供的证据表明，编码消化血液所需酶（胰蛋白酶和凝乳胰蛋白酶样蛋白酶）的基因在滞育雌性中被下调，同时，与脂质储备积累相关的基因（脂肪酸合成酶）被高度上调。当雌性进入滞育期时，脂肪酸合酶只是零星表达，而胰蛋白酶和凝乳胰蛋白酶样的表达仍然无法检测到。在滞育后期（在18摄氏度下2-3个月），编码消化酶的基因开始表达，因为雌性在滞育结束后准备吸血。因此，我们的研究结果强调了一个分子开关，该开关要么使蚊子能够吸血（非滞育），要么使成年蚊子只进行糖摄食和脂质隔离（滞育）。
A key characteristic of overwintering dormancy (diapause) in the mosquito Culex pipiens is the switch in females from blood feeding to sugar gluttony. We present evidence demonstrating that genes encoding enzymes needed to digest a blood meal (trypsin and a chymotrypsin-like protease) are down-regulated in diapause-destined females, and that concurrently, a gene associated with the accumulation of lipid reserves (fatty acid synthase) is highly upregulated. As the females then enter diapause, fatty acid synthase is only sporadically expressed, and expression of trypsin and chymotrypsin-like remains undetectable. Late in diapause (2-3 months at 18 degrees C), the genes encoding the digestive enzymes begin to be expressed as the female prepares to take a blood meal upon the termination of diapause. Our results thus underscore a molecular switch that either capacitates the mosquito for blood feeding (nondiapause) or channels the adult mosquito exclusively toward sugar feeding and lipid sequestration (diapause).