Metamorphosis and collagen-IV-fragments stimulate innate immune response in the greater wax moth, Galleria mellonella
Metamorphosis and collagen-IV-fragments stimulate innate immune response in the greater wax moth, Galleria mellonella
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DOI：
10.1016/j.dci.2006.03.002
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Vilcinskas, Andreas
中科院分区：
文献类型：
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作者：
Altincicek, Boran;Vilcinskas, Andreas
介绍了昆虫发育和免疫之间的一种新联系。瞬时增强的溶菌酶，盖勒霉素和昆虫金属蛋白酶抑制剂的表达被发现在变态的大蜡螟，大蜡螟的发病。使用实时PCR相对定量编码这些抗菌肽的mRNA，记录了它们在末龄幼虫转化为包皮期间以及注射重组间质胶原酶（NIMP-1）或IV型胶原小片段后的诱导表达。还发现后者刺激脂肪体中c-Rel蛋白的核输入，涉及Toll或Imd相关信号通路的激活，以及随后的抗菌肽合成。所获得的结果暗示，降解胶原-IV的微生物金属蛋白酶与入侵的病原体或内源性基质金属蛋白酶有助于降解细胞外基质在变态刺激先天性免疫反应。(c)2006爱思唯尔有限公司版权所有。
A novel link between development and immunity in insects is introduced. Transiently enhanced expression of lysozyme, gallerimycin and the insect metalloproteinase inhibitor was discovered at the onset of metamorphosis of the greater wax moth, Galleria mellonella. Relative quantification of mRNAs encoding for these antimicrobial peptides using real-time PCR documents their induced expression during transformation of last instar larvae into prepupae and upon injection of either recombinant interstitial collagenase (NIMP-1) or small-sized fragments of collagen type IV. The latter were also found to stimulate both nuclear import of c-Rel-proteins in the fat body, implicating activation of Toll or Imd-related signaling pathways, and subsequent synthesis of antimicrobial peptides. Obtained results implicate that degradation of collagen-IV by either microbial metalloproteinases associated with invading pathogens or endogenous matrix metalloproteinases contributing to degradation of extracellular matrix during metamorphosis stimulate innate immune responses. (c) 2006 Elsevier Ltd. All rights reserved.