Anti-Biofilm Activity of a Self-Aggregating Peptide against Streptococcus mutans.
Anti-Biofilm Activity of a Self-Aggregating Peptide against Streptococcus mutans.
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DOI：
10.3389/fmicb.2017.00488
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发表时间：
2017
影响因子：
5.2
通讯作者：
Fikrig E
中科院分区：
文献类型：
--
作者：
Ansari JM;Abraham NM;Massaro J;Murphy K;Smith-Carpenter J;Fikrig E
变形链球菌是龋齿的主要病原体，这在很大程度上是由于其粘附于牙齿并在牙齿表面上产生葡聚糖多糖的分子支架的能力。破坏了S.变异菌生物膜可能有助于破坏导致蛀牙和蛀牙的生物膜群落的建立。在这里，我们提出了一个合成肽P1，来自蜱抗冻蛋白，它显着减少S。变形菌生物膜形成。在结晶紫微孔板测定和使用唾液涂覆的羟基磷灰石盘的体外牙齿模型中，用这种肽孵育细胞使生物膜生物量减少约75%。用肽P1处理的细菌形成不规则的生物膜，其具有不连续的细胞聚集体和易于从表面分离的外聚基质。肽P1可以直接与S.变形细胞，但不具有杀菌活性。抗生物膜活性与溶液中的肽聚集和β-折叠形成相关，并且不同长度或电荷分布的替代合成肽不抑制生物膜。这种抗生物膜肽干扰S.变形菌生物膜的形成和结构，并可能有未来的应用，在防止牙齿上的细菌积累。
Streptococcus mutans is the primary agent of dental cavities, in large part due to its ability to adhere to teeth and create a molecular scaffold of glucan polysaccharides on the tooth surface. Disrupting the architecture of S. mutans biofilms could help undermine the establishment of biofilm communities that cause cavities and tooth decay. Here we present a synthetic peptide P1, derived from a tick antifreeze protein, which significantly reduces S. mutans biofilm formation. Incubating cells with this peptide decreased biofilm biomass by approximately 75% in both a crystal violet microplate assay and an in vitro tooth model using saliva-coated hydroxyapatite discs. Bacteria treated with peptide P1 formed irregular biofilms with disconnected aggregates of cells and exopolymeric matrix that readily detached from surfaces. Peptide P1 can bind directly to S. mutans cells but does not possess bactericidal activity. Anti-biofilm activity was correlated with peptide aggregation and β-sheet formation in solution, and alternative synthetic peptides of different lengths or charge distribution did not inhibit biofilms. This anti-biofilm peptide interferes with S. mutans biofilm formation and architecture, and may have future applications in preventing bacterial buildup on teeth.