High-resolution separations and improved ion production and transmission in metabolomics
High-resolution separations and improved ion production and transmission in metabolomics
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DOI：
10.1016/j.trac.2007.11.003
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发表时间：
2008-03-01
影响因子：
13.1
通讯作者：
Smith, Richard D.
中科院分区：
文献类型：
--
作者：
Metz, Thomas O.;Page, Jason S.;Smith, Richard D.
代谢组学分析的目标是检测和定量尽可能多的样品组分，以确定可用于表征研究样品的化合物或“特征”。当利用电喷雾电离产生离子以通过质谱法（MS）进行分析时，重要的是在离子产生之前有效地分离样品成分，以降低复杂性并使电离抑制最小化，从而扩展测量的动态范围以及代谢组的覆盖范围。类似地，MS入口和接口的优化可以导致增加的测量灵敏度。本文综述了高分辨率液相色谱（LC）分离与改进离子产生和传输的LC-MS为基础的代谢组学的作用。我们把额外的重点放在代谢组覆盖率和样品分析吞吐量之间的妥协。(c)2007爱思唯尔有限公司版权所有。
The goal of metabolomics analyses is detection and quantitation of as many sample components as reasonably possible in order to identify compounds or "features" that can be used to characterize the samples under study. When utilizing electrospray ionization to produce ions for analysis by mass spectrometry (MS), it is important that sample constituents are efficiently separated prior to ion production, in order to reduce complexity and minimize ionization suppression and thereby extend the dynamic range of the measurement, as well as the coverage of the metabolome. Similarly, optimization of the MS inlet and interface can lead to increased measurement sensitivity. This review focuses on the role of high-resolution liquid chromatography (LC) separations in conjunction with improved ion production and transmission for LC-MS-based metabolomics. We place additional emphasis on the compromise between metabolome coverage and sample-analysis throughput. (c) 2007 Elsevier Ltd. All rights reserved.