Brainstem glioma progression in juvenile and adult rats.
Brainstem glioma progression in juvenile and adult rats.
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DOI：
10.3171/jns/2008/109/11/0849
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发表时间：
2008-11
影响因子：
4.1
通讯作者：
Yang SH
中科院分区：
文献类型：
--
作者：
Liu Q;Liu R;Kashyap MV;Agarwal R;Shi X;Wang CC;Yang SH
脑干胶质瘤在儿童中很常见，并且是儿童脑肿瘤中预后最差的。另一方面，脑干胶质瘤在成人中很少见，临床研究表明年轻人和成人之间存在不同的生物学行为。本研究旨在开发年轻和成年大鼠的正交异性C6脑干胶质瘤模型，并研究两个年龄组的肿瘤生物学行为。 C6 神经胶质瘤细胞被立体定向植入年轻或成年雄性大鼠的脑桥中。记录神经系统表现和生存时间。分别通过 Ki-67 免疫组织化学染色和 TUNEL 法测定幼年和成年大鼠脑干胶质瘤的肿瘤增殖和凋亡。年轻和成人脑干胶质瘤在神经系统体征的出现、症状持续时间、生存时间、肿瘤生长模式以及肿瘤增殖和凋亡方面存在显着差异。在患有脑干胶质瘤的成年大鼠中观察到相对局灶性肿瘤，而在幼年大鼠中发现扩散性肿瘤。此外，成年大鼠的脑干胶质瘤比幼年大鼠的脑干胶质瘤增殖较少，细胞凋亡较多。本研究表明，年轻和成年大鼠的 C6 脑干胶质瘤模型在神经学表现和组织病理学方面与人类脑干胶质瘤非常相似。我们的研究结果表明，儿童和成人脑干胶质瘤的不同生长模式和侵袭性可能是由于两个年龄组之间不同的宿主细胞环境所致，因此，有必要进一步研究人类儿童和成人脑干胶质瘤之间不同的宿主反应。
Brainstem gliomas are common in children and have the worst prognosis of any brain tumor in children. On the other hand, brainstem gliomas are rare in adult, and clinical studies have suggested different biological behavior between young and adult. The present study was designed to develop an orthotropic C6 brainstem glioma model in young and adult rats, and to investigate the tumor biological behavior in the two age groups. C6 glioma cells were stereotactically implanted into the pons of young or adult male rats. Neurological presentation and survival time were recorded. Tumor proliferation and apoptosis in brainstem gliomas of young and adult rats were determined by immunohistochemical staining of Ki-67 and TUNEL assay, respectively. Striking difference were found between young and adult brainstem glioma in the onset of neurological signs, duration of symptoms, survival time, tumor growth pattern, as well as tumor proliferation and apoptosis. Relatively focal tumors were observed in adult rats harboring brainstem glioma, while diffusive tumors were found in young rats. Furthermore, brainstem gliomas in adult rats were less proliferative and had more apoptosis than those in young rats. The present study demonstrated that C6 brainstem glioma model in young and adult rats closely imitates human brainstem glioma in neurological findings and histopathology. Our findings suggest that the different growth pattern and invasiveness of brainstem glioma between children and adult could be due to the different host cellular environments between the two age groups, hence, warrant further investigation of the different host-response between childhood and adult brainstem glioma in human.