Integration of programmable microfluidics and on-chip fluorescence detection for biosensing applications
Integration of programmable microfluidics and on-chip fluorescence detection for biosensing applications
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DOI：
10.1063/1.4897226
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发表时间：
2014-09-01
期刊：
影响因子：
3.2
通讯作者：
Schmidt, H.
中科院分区：
文献类型：
--
作者：
Parks, J. W.;Olson, M. A.;Schmidt, H.
我们描述了一个积极控制的可编程微流体样品处理器与芯片上的光学荧光检测，以创建一个单一的，混合的传感器系统的集成。一个升降门微阀（自动机）的阵列制造与软光刻，这是可重构地加入到液芯，抗谐振反射光波导（ARROW）与传统的微加工制造的硅芯片。在自动机中，各种样品处理步骤，如混合，运输，分裂，分离和储存，快速，准确地检测病毒核酸目标，而光流控芯片提供了使用集成光学单粒子检测灵敏度。具体地，实施用于检测病毒核酸靶标的测定。标记的靶核酸首先在自动机中的磁性微珠上捕获和分离，然后在ARROW芯片上对单个珠进行光学检测。自动化微流体样品制备和高灵敏度光学检测的组合为便携式仪器提供了可能性，用于对微小的低浓度生物样品进行使用点分析。(C)2014 AIP Publishing LLC.
We describe the integration of an actively controlled programmable microfluidic sample processor with on-chip optical fluorescence detection to create a single, hybrid sensor system. An array of lifting gate microvalves (automaton) is fabricated with soft lithography, which is reconfigurably joined to a liquid-core, antiresonant reflecting optical waveguide (ARROW) silicon chip fabricated with conventional microfabrication. In the automaton, various sample handling steps such as mixing, transporting, splitting, isolating, and storing are achieved rapidly and precisely to detect viral nucleic acid targets, while the optofluidic chip provides single particle detection sensitivity using integrated optics. Specifically, an assay for detection of viral nucleic acid targets is implemented. Labeled target nucleic acids are first captured and isolated on magnetic microbeads in the automaton, followed by optical detection of single beads on the ARROW chip. The combination of automated microfluidic sample preparation and highly sensitive optical detection opens possibilities for portable instruments for point-of-use analysis of minute, low concentration biological samples. (C) 2014 AIP Publishing LLC.