Inflammatory Responses of Bovine Polymorphonuclear Neutrophils Induced by Staphylococcal Enterotoxin C via Stimulation of Mononuclear Cells
Inflammatory Responses of Bovine Polymorphonuclear Neutrophils Induced by Staphylococcal Enterotoxin C via Stimulation of Mononuclear Cells
复制标题
DOI：
10.1128/cdli.10.6.1011-1018.2003
复制
发表时间：
2003-11
影响因子：
--
通讯作者：
T. Kuroishi;K. Komine;K. Asai;J. Kobayashi;Kouichi Watanabe;Takahiro Yamaguchi;S. Kamata;K. Kumagai
中科院分区：
文献类型：
--
作者：
T. Kuroishi;K. Komine;K. Asai;J. Kobayashi;Kouichi Watanabe;Takahiro Yamaguchi;S. Kamata;K. Kumagai
摘要为了阐明葡萄球菌肠毒素C（SEC）在奶牛葡萄球菌性乳腺炎中的病理作用，对SEC接种的乳腺进行了组织病理学分析。SEC接种的乳腺表现出间质性炎症，迁移到腺体的白细胞主要是多形核中性粒细胞（PMN）。在从SEC接种的乳腺解剖的腺池组织中，观察到上皮细胞变性。我们还研究了SEC在体外对PMN的生理作用。中性粒细胞迁移可被SEC刺激的外周血单个核细胞培养上清（S-PBMC sup）诱导，而不被非刺激的PBMC培养上清（N-PBMC sup）诱导。S-PBMC sup中IL-8浓度显著高于N-PBMC sup（P < 0.05），SEC刺激的PBMC中生长调节癌基因mRNA表达显著高于未刺激的PBMC（P < 0.05）。从SEC接种的乳腺收集的牛奶PMN产生超过2倍的超氧化物的量在接种后1天（dpi）比在0 dpi的佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）的存在下。在PMA存在下，PMN与S-PBMC sup共同培养24 h后，其超氧化物歧化酶的产生量也显著高于与N-PBMC sup共同培养24 h后的PMN（P < 0.05）。此外，S-PBMC可诱导PMN的长期存活。这些结果表明SEC通过刺激单个核细胞诱导PMN活化。
ABSTRACT To elucidate the pathological roles of staphylococcal enterotoxin C (SEC) in bovine staphylococcal mastitis, a histopathological analysis of SEC-inoculated mammary glands was performed. SEC-inoculated mammary glands exhibited interstitial inflammation, and the leukocytes that migrated into the gland were predominantly polymorphonuclear neutrophils (PMN). In the gland cistern tissues dissected from SEC-inoculated mammary glands, epithelial cellular degeneration was observed. We also investigated the physiological effects of SEC on PMN in vitro. PMN migration was induced by culture supernatant of SEC-stimulated peripheral blood mononuclear cells (S-PBMC sup) but not by that of nonstimulated PBMC (N-PBMC sup). The concentration of interleukin-8 was significantly (P < 0.05) higher in S-PBMC sup than N-PBMC sup, and a significantly (P < 0.05) higher mRNA expression of growth-regulated oncogenes was detected in SEC-stimulated PBMC than in nonstimulated PBMC. Milk PMN collected from SEC-inoculated mammary glands produced more than 2 times the amount of superoxide at 1 day postinoculation (dpi) than at 0 dpi in the presence of phorbol 12-myristate 13-acetate (PMA). PMN cultured with S-PBMC sup for 24 h also produced significantly (P < 0.05) larger amounts of superoxide than those cultured with N-PBMC sup in the presence of PMA. Moreover, S-PBMC sup induced the long-time survival of PMN. These results indicate that SEC induces the activation of PMN via the stimulation of mononuclear cells.