Gingiva-Derived Mesenchymal Stem Cell-Mediated Therapeutic Approach for Bone Tissue Regeneration
Gingiva-Derived Mesenchymal Stem Cell-Mediated Therapeutic Approach for Bone Tissue Regeneration
复制标题
牙龈源性间充质干细胞介导的骨组织再生治疗方法
DOI：
10.1089/scd.2010.0523
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发表时间：
2011-12-01
影响因子：
4
通讯作者：
Pei, Xuetao
中科院分区：
文献类型：
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作者：
Wang, Fang;Yu, Meijiao;Pei, Xuetao
目前，牙周间充质干细胞(GMSCs)作为一种新的出生后干细胞已被分离并具有多向分化潜能。然而，利用GMSCs进行骨再生的体内效果仍不清楚。首先，我们从人牙龈组织中鉴定出具有免疫表型(CD34(-)CD45(-)CD29(+)CD105(+)CD90(+)Stro-1(+))并具有三系分化潜能(成骨细胞、脂肪细胞和软骨细胞)的典型MSCs。接下来，我们检测了这些干细胞在骨组织再生中的作用；将增强型绿色荧光蛋白标记的GMSCs种植在I型胶原凝胶上，植入SD大鼠的下颌骨缺损和临界大小的颅骨缺损中。我们首次在大鼠下颌骨创面和颅骨重建术后2个月时证实了GMSCs可以修复下颌骨创面和颅骨缺损。组织形态分析和荧光显微镜图像分析证实，缺损区的新骨来源于移植的GMSCs。绿色荧光蛋白、人I型胶原和骨桥蛋白的免疫组织化学分析进一步证实了我们的结论。以上结果提示，牙周组织来源的间充质干细胞有可能成为骨重建中干细胞治疗的一种新来源。
Up to now, the gingiva-derived mesenchymal stem cells (GMSCs) as a new postnatal stem cells have been isolated and characterized with multipotential differentiation capabilities in vitro. However, the in vivo efficacy of utilizing the GMSCs in bone regeneration remains obscure. First of all, we identified canonical MSCs in human gingival tissue, which possessed homogenous immunophenotype (CD34(-)CD45(-)CD29(+)CD105(+)CD90(+) STRO-1(+)) and had tri-lineage differentiation potential (osteoblasts, adipocytes, and chondrocytes). Next, we examined the efficacy of utilizing these stem cells in bone tissue regeneration; the enhanced green fluorescent protein-labeled GMSCs seeded on type I collagen gel were implanted into the mandibular defects as well as the critical-sized calvarial defects in Sprague Dawley rats. We first demonstrated that GMSCs could repair the mandibular wounds and calvarial defects at 2 months in rats postsurgical reconstruction. Histomorphological analysis and image of fluorescence microscope certified that new bone in the defect areas was derived from the transplanted GMSCs. Immunohistochemical analysis of green fluorescent protein, human collagen I, and osteopontin further confirmed our conclusion. The above results implied that mesenchymal stem cells derived from gingival tissue could be a novel source for stem cell-based therapy in bone reconstruction in clinical applications.