Liquid chromatographic assay for the simultaneous determination of indole-3-carbinol and its acid condensation products in plasma
Liquid chromatographic assay for the simultaneous determination of indole-3-carbinol and its acid condensation products in plasma
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DOI：
10.1016/s1570-0232(02)00923-6
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发表时间：
2003-04-25
影响因子：
3
通讯作者：
Williams, ML
中科院分区：
文献类型：
--
作者：
Anderton, MJ;Jukes, R;Williams, ML
建立了同时测定吲哚-3-甲醇(I3C)、3，3‘-二吲哚甲烷(DIM)、吲哚并[3，2b]咔唑([2-(indol-3-ylmethyl)-indol-3-yl]indol-3-ylmethane)和吲哚并[3，2b]咔唑(ICZ)的高效液相色谱方法。以4-甲氧基吲哚为内标，叔丁基甲醚为内标，从小鼠血浆中提取化合物。使用Waters SymmetryRP18色谱柱与ThermoquestBDS C-18色谱柱、乙腈-水梯度色谱柱和紫外光(280 Nm)串联荧光(例如。335 nm；415 nm)检测。I3C在50~15,000 ng/ml，LTr1在150~15,000 ng/ml，ICZ在0.15~37.5 ng/ml范围内线性关系良好(r(2&>)0.99)，方法重现性好，精密度好(日内和日间变异系数分别低于9.7%和13%)。该方法适用于吲哚-3-甲醇的综合药动学研究。(C)2002 Elsevier Science B.V.保留所有权利。
A high-performance liquid chromatographic method was developed for the simultaneous determination of indole-3-carbinol (I3C), 3,3' diindolylmethane (DIM), [2-(indol-3-ylmethyl)-indol-3-yl]indol-3-ylmethane (LTr1), and indolo[3,2b]carbazole (ICZ). Compounds were extracted from mouse plasma using tert.-butyl methyl ether, incorporating 4-methoxy-indole as internal standard. Chromatographic separation utilized a Waters Symmetry RP18 in tandem with a Thermoquest BDS C-18 column, an acetonitrile-water gradient and UV (280 nm) in series with fluorescence (ex. 335 nm; em. 415 nm) detection. Calibration curves were linear (r(2>)0.99) between 50 and 15,000 ng/ml for I3C; 150 and 15,000 ng/ml for LTr1; and 0.15 and 37.5 ng/ml for ICZ and the method was reproducible and precise (within-day and between-day coefficients of variation below 9.7 and 13%, respectively). The method described is suitable for comprehensive pharmacokinetic studies with indole-3-carbinol. (C) 2002 Elsevier Science B.V. All rights reserved.