A pathological study of the tongues of rabid dogs in the Philippines
A pathological study of the tongues of rabid dogs in the Philippines
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DOI：
10.1007/s00705-018-3785-y
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发表时间：
2018-06-01
影响因子：
2.7
通讯作者：
Park, Chun-Ho
中科院分区：
文献类型：
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作者：
Shiwa, Nozomi;Kimitsuki, Kazunori;Park, Chun-Ho
在狂犬病病毒感染过程中，小唾液腺是病毒复制和排泄到口腔中的重要器官之一。然而，详细的病理结果和病毒抗原分布的小唾液腺仍然知之甚少。在这项研究中，我们在菲律宾的71只狂犬病犬的舌头上进行了病理学测试，小唾液腺（von Ebner腺，舌腺），轮廓乳头，自主神经节和骨骼肌进行了评价。在20/71只犬的von Ebner腺、10/71只犬的轮廓乳头和1/71只犬的舌肌中观察到炎症变化。相反，在舌腺和自主神经节中未观察到形态学变化。在所有71只犬的von Ebner腺腺泡上皮细胞的细胞质中，通过基于免疫化学的方法检测到病毒抗原。电镜下可见病毒颗粒存在于细胞间小管和腺泡腔内。在自主神经节中，在67/71只狂犬病犬中检出病毒抗原。在71只犬的味蕾中均检测到病毒抗原，主要分布在II型和III型味蕾细胞中。在舌肌纤维中，在11/71只狗中检测到病毒抗原。舌腺中未检测到病毒抗原。这些发现表明狂犬病毒在脑中增殖后沿着舌咽神经在舌中下降，并且von Ebner腺和味蕾是狂犬病犬将病毒排泄到唾液中的门户之一。
During rabies virus infections, the minor salivary glands are one of the important organs for virus replication and excretion into the oral cavity. However, details of pathological findings and viral antigen distribution in the minor salivary glands remain poorly understood. In this study, we conducted pathological tests on the tongues of 71 rabid dogs in the Philippines; the minor salivary glands (von Ebner's glands, lingual glands), circumvallate papilla, autonomic ganglia, and skeletal muscles were evaluated. Inflammatory changes were observed in the von Ebner's glands of 20/71 dogs, in the circumvallate papilla of 10/71, and in the tongue muscle of 1/71. Conversely, no morphological changes were observed in the lingual glands and autonomic ganglia. Viral antigens were detected via immunohistochemistry-based methods in the cytoplasm of the acinar epithelium in the von Ebner's glands of all 71 dogs. Virus particles were confirmed in the intercellular canaliculi and acinar lumen via electron microscopy. In the autonomic ganglia, viral antigens were detected in 67/71 rabid dogs. Viral antigens were detected in the taste buds of all 71 dogs, and were distributed mainly in type II and III taste bud cells. In tongue muscle fibers, viral antigens were detected in 11/71 dogs. No virus antigens were detected in lingual glands. These findings suggest that rabies virus descends in the tongue along the glossopharyngeal nerve after proliferation in the brain, and von Ebner's glands and taste buds are one of the portals of virus excretion into the saliva in rabid dogs.