Serum sulfatide abnormality is associated with increased oxidative stress in hemodialysis patients
Serum sulfatide abnormality is associated with increased oxidative stress in hemodialysis patients
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血液透析患者血清硫苷脂异常与氧化应激增加相关
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
T. Aoyama
中科院分区：
文献类型：
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作者：
Huang Yuzhe;Y. Kamijo;Koji Hashimoto;M. Harada;Taro Kanno;E. Sugiyama;M. Kyogashima;T. Oguchi;Takero Nakajima;Y. Kanno;T. Aoyama
脑硫脂是脂蛋白的主要糖脂，影响动脉粥样硬化和凝血。我们之前对血液透析患者的横断面研究表明，随着血液透析治疗时间的延长，血清硫苷脂水平显着下降，这可能有助于心血管疾病的发生。然而，过去的研究无法证明每位患者血清硫苷脂水平随时间的变化，且其潜在机制尚不清楚。为了证实血清硫苷脂异常的时间依赖性恶化，对 95 名稳定的血液透析门诊患者进行了为期 3 年的随访。为了揭示潜在机制，我们统计分析了血清硫苷脂水平与临床因素（包括氧化应激标记物丙二醛）之间的相关性。血清硫苷脂转化为溶血硫苷脂后通过质谱法进行定量。使用比色测定法测量丙二醛。结果显示，血清硫苷脂水平呈时间依赖性下降，与丙二醛水平升高相关，尽管血清丙二醛的绝对水平并不能确定血清硫苷脂的基线水平。多元线性回归分析显示，仅血清硫苷脂水平的时间依赖性变化与血清丙二醛水平的时间依赖性变化之间存在显着相关性。该研究首次证明了血液透析患者血清硫苷脂异常随时间的加重，以及血清硫苷脂异常与氧化应激增加之间的潜在关系。这些发现表明氧化应激可能是血清硫苷脂异常的加重因素。由于继续血液透析治疗很难改善异常的血清硫苷脂水平或增加的氧化应激，因此开发新的治疗策略可能很重要。
Sulfatides are major glycosphingolipids of lipoproteins that influence atherosclerosis and blood coagulation. Our previous cross‐sectional study of hemodialysis patients showed that serum sulfatide levels decreased markedly with increasing duration of hemodialysis treatment, which may contribute to the development of cardiovascular disease. However, this past study could not demonstrate the time‐dependent change in serum sulfatide levels in each patient, and the underlying mechanism is unknown. To confirm the time‐dependent aggravation of serum sulfatide abnormality, 95 stable hemodialysis outpatients were followed up for 3 years. To show the underlying mechanisms, we statistically analyzed correlations between serum sulfatide levels and clinical factors, including an oxidative stress marker, malondialdehyde. Serum sulfatides were quantified by mass spectrometry after conversion to lysosulfatides. Malondialdehyde was measured using a colorimetric assay. The results showed a time‐dependent decrease in serum sulfatide levels associated with increased malondialdehyde levels, although the absolute level of serum malondialdehyde does not determine the baseline level of serum sulfatides. Multiple linear regression analysis showed a significant correlation only between the time‐dependent change in serum sulfatide levels and the time‐dependent change in serum malondialdehyde levels. This study demonstrated, for the first time, a time‐dependent aggravation of serum sulfatide abnormality in hemodialysis patients, as well as the potential relationship between serum sulfatide abnormality and increasing oxidative stress. These findings suggest that oxidative stress might be an aggravating factor in serum sulfatide abnormality. As continuation of hemodialysis treatment hardly improves abnormal serum sulfatide levels or increased oxidative stress, development of novel therapeutic strategies may be important.
DOI：
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发表时间：
1989-06
期刊：
The Journal of biological chemistry
影响因子：
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作者：
T. Aoyama;S. Yamano;D. Waxman;D. Lapenson;U. Meyer;V. Fischer;R. Tyndale;T. Inaba;W. Kalow
通讯作者：
T. Aoyama;S. Yamano;D. Waxman;D. Lapenson;U. Meyer;V. Fischer;R. Tyndale;T. Inaba;W. Kalow