A PATHOGEN-INDUCED WHEAT GENE ENCODES A PROTEIN HOMOLOGOUS TO GLUTATHIONE-S-TRANSFERASES
A PATHOGEN-INDUCED WHEAT GENE ENCODES A PROTEIN HOMOLOGOUS TO GLUTATHIONE-S-TRANSFERASES
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DOI：
10.1094/mpmi-4-014
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发表时间：
1991-01-01
影响因子：
3.5
通讯作者：
MAUCH, F
中科院分区：
文献类型：
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作者：
DUDLER, R;HERTIG, C;MAUCH, F
冬小麦（Triticum aestivum）在暴露于非致病性真菌（如hordei. sp.）后，对植物致病性真菌（如graminis . sp.）表现出局部诱导抗性。这种抗性的发生与防御基因的激活有关，并且获得了代表诱导基因转录本的cDNA克隆（P. Schweizer， W. Hunziker, and E. Mosinger, Plant Molecular Biology, 1989:643-654）。我们已经克隆并测序了一个与这些cdna之一WIR5相对应的基因。序列分析表明，该基因含有3个外显子，编码229个氨基酸的蛋白。S1图谱显示，在早14小时被霍代赤霉病菌侵染的叶片中，与该基因同源的转录本的丰度至少是对照叶片的20倍。序列比较表明，WIR5基因产物与玉米谷胱甘肽- s转移酶（GSTs; EC 25.1.18）高度同源。这一点，再加上小麦基因和玉米GSTI基因的内含子位置都是保守的，表明克隆的病原体诱导基因GstA1编码小麦谷胱甘肽- s转移酶。
Winter wheat (Triticum aestivum) shows local, induced resistance against the plant-pathogenic fungus Erysiphe graminis f. sp. tritici following exposure to the nonpathogen E. g. f. sp. hordei. The onset of this resistance has been shown to be correlated with the activation of putative defense genes, and cDNA clones representing transcripts of induced genes have been obtained (P. Schweizer, W. Hunziker, and E. Mosinger, Plant Molecular Biology 12:643-654, 1989). We have cloned and sequenced a gene corresponding to one of these cDNAs, WIR5. Sequence analysis indicated that this gene contains three exons and encodes a protein of 229 amino acids. S1 mapping showed that transcripts homologous to this gene are at least 20 times more abundant in leaves infected 14 hr earlier with E. g. f. sp. hordei than in control leaves. Sequence comparison showed that the WIR5 gene product is highly homologous to glutathione-S-transferases (GSTs; EC 25.1.18) of maize. This, together with the fact that the intron positions of both the wheat gene and the maize GSTI gene are conserved, suggests that the cloned pathogen-induced gene, named GstA1, encodes a wheat glutathione-S-transferase.