Sphingolipids as a new factor in the pathomechanism of preeclampsia - Mass spectrometry analysis.
Sphingolipids as a new factor in the pathomechanism of preeclampsia - Mass spectrometry analysis.
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DOI：
10.1371/journal.pone.0177601
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Laudanski P
中科院分区：
文献类型：
--
作者：
Charkiewicz K;Goscik J;Blachnio-Zabielska A;Raba G;Sakowicz A;Kalinka J;Chabowski A;Laudanski P
这项研究的目的是分析轻度子痫前期妇女血浆和三种血液成分(缺乏血小板的血浆、血小板和红细胞)中的11种鞘磷脂。我们招募了21例妊娠25-40周诊断为轻度子痫前期的妇女为研究组，36例无并发症妊娠的健康妇女为对照组，按胎龄与研究组相对应。为了评估11种鞘磷脂在血浆和血液组份中的浓度，我们使用了超高效液相色谱-三重四极杆(UHPLC/MS/MS)联用技术。与对照组相比，子痫前期患者血浆中8种鞘磷脂的浓度显著升高：Sph(p=0.0032)、S1P(p=0.0289)、C20-Cer(p<；0.0001)、C18-Cer(p<；0.0001)、C16-Cer(p=0.012)、C18：1-Cer(p=0.003)、C22-Cer(p=0.0071)和C24：1-Cer(p=0.0085)。我们发现选择性的鞘磷脂，特别是C20-Cer和C18-Cer，在PE的发病机制中是全新的因子，这些生物活性脂类可能在细胞凋亡和自噬中发挥重要作用。
The aim of the study was to analyse a panel of 11 sphingolipids in plasma and three blood fractions (platelet-poor plasma, platelets and red blood cells) of women with mild preeclampsia. We recruited 21 women between 25–40 weeks gestation with diagnosed mild preeclampsia to the study group and 36 healthy women with uncomplicated pregnancies, who corresponded with the study group according to gestational age, to the control group. To assess the concentration of 11 sphingolipids in the blood plasma and blood fractions, we used ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC/MS/MS). We showed a significant increase in the concentration of eight sphingolipids in the plasma of women with preeclampsia in comparison to the control group: Sph (p = 0.0032), S1P (p = 0.0289), C20-Cer (p < 0.0001), C18-Cer (p < 0.0001), C16-Cer (p = 0.012), C18:1-Cer (p = 0.003), C22-Cer (p = 0.0071), and C24:1-Cer (p = 0.0085). We showed that selected sphingolipids, especially C20-Cer and C18-Cer, are totally new factors in the pathomechanism of PE and that these bioactive lipids may play an important role in apoptosis and autophagy.