A sprayable luminescent pH sensor and its use for wound imaging in vivo
A sprayable luminescent pH sensor and its use for wound imaging in vivo
复制标题
DOI：
10.1111/exd.12042
复制
发表时间：
2012-12-01
影响因子：
3.6
通讯作者：
Babilas, Philipp
中科院分区：
文献类型：
--
作者：
Schreml, Stephan;Meier, Robert J.;Babilas, Philipp
无创发光成像在伤口愈合、肿瘤等生物医学领域的生物学参数研究中具有重要意义。最近，我们开发了第一种人体体内pH值的二维发光成像方法，以及一种利用数码相机的RGB读出氧和pH值的一站式可视化新方法。这两种方法都使用了半透明的传感器箔。在这里，我们描述了一种可喷涂的比率发光pH传感器，它结合了这两种方法的特性。此外，一个主要的优点是，由于其一致性，传感器喷雾适用于非常不均匀的组织表面。在组织上拍摄喷雾剂的数字RGB图像。pH指示剂（异硫氰酸荧光素）的信号存储在绿色通道(G)中，而参考染料[钌(II)-三-（4,7-二苯基-1,10-菲罗啉）]的信号存储在红色通道(R)中。通过定量发光强度（G/R）对图像进行处理，生成组织（如伤口）的伪彩色pH图。
Non-invasive luminescence imaging is of great interest for studying biological parameters in wound healing, tumors and other biomedical fields. Recently, we developed the first method for 2D luminescence imaging of pH in vivo on humans, and a novel method for one-stop-shop visualization of oxygen and pH using the RGB read-out of digital cameras. Both methods make use of semitransparent sensor foils. Here, we describe a sprayable ratiometric luminescent pH sensor, which combines properties of both these methods. Additionally, a major advantage is that the sensor spray is applicable to very uneven tissue surfaces due to its consistency. A digital RGB image of the spray on tissue is taken. The signal of the pH indicator (fluorescein isothiocyanate) is stored in the green channel (G), while that of the reference dye [ruthenium(II)-tris-(4,7-diphenyl-1,10-phenanthroline)] is stored in the red channel (R). Images are processed by rationing luminescence intensities (G/R) to result in pseudocolor pH maps of tissues, e.g. wounds.