Uncoupling of sister replisomes during eukaryotic DNA replication.
Uncoupling of sister replisomes during eukaryotic DNA replication.
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DOI：
10.1016/j.molcel.2010.11.027
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发表时间：
2010-12-10
期刊：
影响因子：
16
通讯作者：
Walter JC
中科院分区：
文献类型：
--
作者：
Yardimci H;Loveland AB;Habuchi S;van Oijen AM;Walter JC
真核生物基因组的复制涉及来自多达数十万个复制起点的DNA复制。在S期，两个姐妹复制体在每个活性起点组装，它们以相反的方向复制DNA。S期姐妹复制体之间的功能关系知之甚少。一些数据表明，它们彼此远离，因此独立运作。然而，其他研究表明，姐妹复制体形成一个固定的功能单位，将亲本DNA吸引到自己身上。如果这种“双复制体”模型是正确的，那么末端固定在空间中的延伸DNA分子就不应该进行复制。为了测试该预测，将λ DNA（50 kb）拉伸并固定在微流体流动池内的两端。在暴露于具有复制能力的爪蟾卵提取物后，该DNA通过一对不同的复制体进行了广泛的复制。这些数据表明，姐妹复制体之间没有强制性的偶联，并且与其他研究一起，暗示基因组复制是由个体自主功能的复制体进行的。
The duplication of eukaryotic genomes involves the replication of DNA from up to several hundred thousand origins of replication. In S phase, two sister replisomes are assembled at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes in S phase. Some data imply that they travel away from one another and thus function independently. However, other studies suggest that sister replisomes form a stationary, functional unit that draws parental DNA towards itself. If this ‘double replisome’ model is correct, an extended DNA molecule whose ends are fixed in space should not undergo replication. To test this prediction, lambda DNA (50 kb) was stretched and immobilized at both ends within a microfluidic flow cell. Upon exposure to replication-competent Xenopus egg extracts, this DNA underwent extensive replication by a single pair of diverging replisomes. The data show that there is no obligatory coupling between sister replisomes and, together with other studies, imply that genome duplication is carried out by individual, autonomously functioning replisomes.
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影响因子：
21.3
作者：
Gambus, A;Jones, RC;Labib, K
通讯作者：
Labib, K
DOI：
10.1073/pnas.0911500106
发表时间：
2009-12-01
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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