Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells.
Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells.
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DOI：
10.1007/978-1-4939-6820-6_3
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Rane SG
中科院分区：
文献类型：
--
作者：
Wankhade UD;Rane SG
脂肪组织功能障碍通常见于代谢疾病，特别是肥胖和糖尿病。白色脂肪细胞储存脂肪，而棕色脂肪细胞通过产热作用消散脂肪。此外，米色脂肪细胞在白色脂肪库中发育，以响应β-肾上腺素能途径的刺激。三种类型的脂肪细胞——白色、棕色和米色——似乎可以由干细胞/前体细胞或通过转分化从头形成。鉴定具有分化为这些不同脂肪细胞类型的能力的假定祖细胞将能够表征它们的功能。此外，白色/棕色/米色脂肪细胞的存在或不存在与代谢功能障碍相关，这使得他们的研究具有医学意义。稳健、可靠和可重复的脂肪细胞祖细胞鉴定和分离方法将促进对白色、棕色和米色脂肪生成的进一步详细了解。
Adipose tissue dysfunction is typically seen in metabolic diseases, particularly obesity and diabetes. White adipocytes store fat while brown adipocyte dissipates it via thermogenesis. In addition, beige adipocytes develop in white fat depots in response to stimulation of β-adrenergic pathways. It appears that the three types of adipocytes – white, brown and beige – can be formed de novo from stem/precursor cells or via transdifferentiation. Identifying the presumptive progenitors that harbor capacity to differentiate to these distinct adipocyte cell types will enable their functional characterization. Moreover the presence or absence of white/brown/beige adipocytes is correlated with metabolic dysfunction making their study of medical relevance. Robust, reliable and reproducible methods of identification and isolation of adipocyte progenitors will stimulate further detailed understanding of white, brown and beige adipogenesis.