The high-affinity zinc-uptake system znuA CB is under control of the iron-uptake regulator (fur) gene in the animal pathogen Pasteurella multocida
The high-affinity zinc-uptake system znuA CB is under control of the iron-uptake regulator (fur) gene in the animal pathogen Pasteurella multocida
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DOI：
10.1016/s0378-1097(03)00131-9
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发表时间：
2003-04-11
影响因子：
2.1
通讯作者：
Barbé, J
中科院分区：
文献类型：
--
作者：
Garrido, ME;Bosch, M;Barbé, J
编码高亲和力锌吸收系统的多杀性巴氏杆菌znuACB基因已被鉴定和克隆。与大肠杆菌中发生的情况相反，znuA与znuCB没有物理联系。通过lacZ转录融合，已经证明锌负调控znuA和znuCB操纵子。然而，与迄今为止已知的所有其他znuACB细菌系统所确定的相反，多杀巴斯德菌znuACB基因不受zur基因的控制，zur基因在该细菌物种中不存在，而是在其铁摄取调节因子（fur）基因的控制下。此外，缺陷突变体的构建已经证明，多杀性巴氏杆菌znuA和znuCB转录单位是该生物体在小鼠模型中的毒力所需的。(C)2003年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
The Pasteurella multocida znuACB genes encoding a high-affinity zinc-uptake system have been identified and cloned. In contrast to what happens in Escherichia coli, znuA is not physically linked to znuCB. Through lacZ transcriptional fusions it has been demonstrated that zinc negatively regulates both znuA and znuCB operons. Nevertheless, and, contrary to that determined so far for all other znuACB bacterial systems known, P. multocida znuACB genes are not under control of the zur gene, which is absent in this bacterial species, but rather are under its iron-uptake regulator (fur) gene. Furthermore, construction of defective mutants has, demonstrated that P. multocida znuA and znuCB transcriptional units are required for virulence of this organism in a mouse model. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.