Changes in gene expression associated with developmental arrest and longevity in Caenorhabditis elegans
Changes in gene expression associated with developmental arrest and longevity in Caenorhabditis elegans
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DOI：
10.1101/gr.184401
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发表时间：
2001-08-01
期刊：
影响因子：
7
通讯作者：
Marra, MA
中科院分区：
文献类型：
--
作者：
Jones, SJM;Riddle, DL;Marra, MA
采用基因表达系列分析（SAGE）技术，比较了秀丽隐杆线虫发育停滞、长寿的dauer种群与非dauer（混合期）种群的基因表达。Dauer（152，314）和nondauer（148，324）SAGE标签鉴定了预测的19，100个秀丽隐杆线虫基因中的11，130个。先前与长寿有关的基因在道尔文库中大量表达，并发现了在道尔生物学中潜在重要的新基因。仅在非道尔种群中检测到2618个基因，而仅在道尔种群中检测到2016个基因，表明道尔幼虫显示出令人惊讶的复杂基因表达谱。差异表达的基因转录异构体的证据，获得了162个基因。Hi组蛋白。差异表达，提高了替代染色质包装的可能性。从dauer幼虫中获得的最丰富的标签（比非dauer图谱中的丰富20倍）对应于一个新的，我们命名为tts-1（转录的端粒样序列）的未预测基因，它可能与端粒或端粒相关蛋白相互作用。丰富的反义线粒体转录本（占所有标签的2%）表明秀丽隐杆线虫线粒体中存在反义介导的调节机制。除了为基因表达研究提供了一个强大的工具，SAGE方法已经提供了在后生动物中发现新基因/转录本的优势。
Gene expression in a developmentally arrested, long-lived dauer Population of Caenorhabditis elegans was compared with a nondauer (mixed-stage) population by using serial analysis of gene expression (SAGE). Dauer (152,314) and nondauer (148,324) SAGE tags identified If,130 of the predicted 19,100 C elegans genes. Genes implicated previously in longevity were expressed abundantly in the dauer library, and new genes potentially important in dauer biology were discovered. Two thousand six hundred eighteen genes were detected only in the nondauer population, whereas 2016 genes were detected only in the dauer, showing that dauer larvae show a surprisingly complex gene expression profile. Evidence for differentially expressed gene transcript isoforms was obtained for 162 genes. HI histones. were differentially expressed, raising the possibility of alternative chromatin packaging. The most abundant tag from dauer larvae (20-fold more abundant than in the nondauer profile) corresponds to a new, unpredicted gene we have named tts-1 (transcribed telomere-like sequence), which may interact with telomeres or telomere-associated proteins. Abundant antisense mitochondrial transcripts (2% of all tags), suggest the existence of an antisense-mediated: regulatory mechanism in C elegans mitochondria. In addition to providing a robust tool for gene expression studies, the SAGE approach already has provided the advantage of new gene/transcript discovery in a metazoan.