PIPERINE, A MAJOR INGREDIENT OF BLACK AND LONG PEPPERS, PROTECTS AGAINST AFB(1)-INDUCED CYTOTOXICITY AND MICRONUCLEI FORMATION IN H4IIEC3 RAT HEPATOMA-CELLS
PIPERINE, A MAJOR INGREDIENT OF BLACK AND LONG PEPPERS, PROTECTS AGAINST AFB(1)-INDUCED CYTOTOXICITY AND MICRONUCLEI FORMATION IN H4IIEC3 RAT HEPATOMA-CELLS
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DOI：
10.1016/0304-3835(94)90078-7
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发表时间：
1994-11-11
期刊：
影响因子：
9.7
通讯作者：
WIEBEL, FJ
中科院分区：
文献类型：
--
作者：
SINGH, J;REEN, RK;WIEBEL, FJ
以细胞生长和微核形成为指标，研究了胡椒碱对黄曲霉毒素B-1（AFB（1））的细胞毒性和遗传毒性的影响。早先显示，在这些细胞的制备物中，苦参碱抑制细胞色素P-450依赖性芳烃羟化酶和7-甲氧基香豆素脱甲基酶的活性，在30-50 μ M时具有1/2的最大抑制作用（Singh J.和Reen R.K.，当代科学，66，365-369，1994）。本研究的结果表明，黄曲霉毒素B（1）抑制H4 IIE细胞的生长，艾德（50）为15 nM。马槟榔碱明显降低了霉菌毒素的毒性。因此，在100 μ M时胡椒碱大大恢复了细胞的生长速率。同样，胡椒碱以浓度依赖性方式减少AFB（1）诱导的微核形成。当浓度高达100 μ M时，苦参碱本身对细胞没有毒性。结果表明，胡椒碱能够通过抑制细胞色素P-450介导的真菌毒素的生物活化来对抗AFB（1）的毒性。
We studied the effect of piperine on the cytotoxicity and genotoxicity of aflatoxin B-1 (AFB(1)) in rat hepatoma cells H4IIEC3/G-(H4IIE) using cellular growth and formation of micronuclei as endpoints. Piperine was earlier shown to inhibit cytochrome P-450-dependent aryl hydrocarbon hydroxylase and 7-methoxycoumarin demethylase activities in preparations of these cells with 1/2 maximum inhibition at 30-50 mu M (Singh J. and Reen R.K., Current Science, 66, 365-369, 1994). The results of the present study showed that AFB(1) inhibited the growth of H4IIE cells with an ED(50) of 15 nM. Piperine markedly reduced the toxicity of the mycotoxin. Thus at 100 mu M piperine largely restored the rate of growth of the cells. Likewise, piperine reduced the AFB(1)-induced formation of micronuclei in a concentration-dependent manner. Piperine itself was not toxic to the cells up to a concentration of almost 100 mu M. The results suggest, that piperine is capable of counteracting AFB(1) toxicity by suppressing cytochromes P-450 mediated bioactivation of the mycotoxin.