Astrocyte growth is regulated by neuropeptides through Tis 8 and basic fibroblast growth factor.
Astrocyte growth is regulated by neuropeptides through Tis 8 and basic fibroblast growth factor.
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星形胶质细胞的生长受神经肽通过 Tis 8 和碱性成纤维细胞生长因子的调节。
DOI：
10.1172/jci117167
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Levin,ER
中科院分区：
文献类型：
--
作者：
Hu,RM;Levin,ER
星形胶质细胞生长的重要细胞内机制尚不清楚。利用星形胶质细胞增殖抑制剂，心房利钠肽（ANP）和胶质有丝分裂原内皮素（ET-3），我们寻找这些神经细胞生长调节的共同途径。在培养的胎鼠间脑星形胶质细胞中，ANP选择性地、快速地抑制Tis 8即时早期基因和蛋白的表达。4 h后，ANP选择性抑制碱性成纤维细胞生长因子（bFGF）基因和蛋白。ET-3显著刺激了Tis - 8和bFGF mrna和蛋白，但也刺激了其他几个即时早期和生长因子/受体基因。Tis 8的反义寡核苷酸强烈阻止et刺激的胸腺嘧啶掺入，而ANP的抑制作用增强。Tis 8反义寡核苷酸也显著逆转et刺激的bFGF转录，增强ANP对bFGF的抑制作用。此外，bFGF的反义寡核苷酸显著逆转了et刺激的胸苷结合，增强了ANP对DNA合成的抑制作用。Tis - 8的顺序调控，以及bFGF的顺序调控，为内源性神经肽对星形胶质细胞生长的正、负调控提供了一种新的机制。图片
The important intracellular mechanisms of astrocyte growth are not well defined. Using an inhibitor of astrocyte proliferation, atrial natriuretic peptide (ANP), and the glial mitogen endothelin (ET-3), we sought a common pathway for growth regulation in these neural cells. In cultured fetal rat diencephalic astrocytes, ANP selectively and rapidly inhibited the Tis 8 immediate early gene and protein. After 4 h, ANP selectively inhibited the basic fibroblast growth factor (bFGF) gene and protein. ET-3 significantly stimulated both Tis 8 and bFGF mRNAs and protein, but also stimulated several other immediate early and growth factor/receptor genes. An antisense oligonucleotide to Tis 8 strongly prevented ET-stimulated thymidine incorporation, while the inhibitory action of ANP was enhanced. The Tis 8 antisense oligonucleotide also significantly reversed ET-stimulated bFGF transcription and enhanced the bFGF inhibition caused by ANP. In addition, an antisense oligonucleotide to bFGF significantly reversed the ET-stimulated thymidine incorporation and enhanced the ANP inhibition of DNA synthesis. The sequential modulation of Tis 8, followed by bFGF, provides a novel mechanism for both positive and negative regulation of astrocyte growth by endogenous neuropeptides.Images