Molecular recognition, fluorescence sensing, and biological assay of phosphate anion derivatives using artificial Zn(II)-Dpa complexes
Molecular recognition, fluorescence sensing, and biological assay of phosphate anion derivatives using artificial Zn(II)-Dpa complexes
复制标题
DOI：
10.1039/b812374h
复制
发表时间：
2009-01-01
影响因子：
4.9
通讯作者：
Hamachi, Itaru
中科院分区：
文献类型：
--
作者：
Sakamoto, Takashi;Ojida, Akio;Hamachi, Itaru
在这篇专题文章中，我们重点介绍了我们在具有生物重要性的磷酸根阴离子衍生物的分子识别和荧光传感方面的最新研究进展。由于它们在生物系统中的重要作用，已经投入了相当大的努力来开发检测或测定系统。然而，这些阴离子物种在水相生物条件下使用小分子化学传感器的识别和传感仍然是一个具有挑战性的研究课题。近年来，我们一直在开发各种磷蛋白和多磷酸核苷的人工受体和荧光化学传感器。它们由双核Zn（II）-吡啶二甲基胺（Dpa）络合物作为磷酸根阴离子衍生物的共同结合基序组成。利用它们的强结合亲和力或高传感能力，各种生物测定系统也已成功开发，其中包括酶测定，如激酶，磷酸酶和糖基转移酶反应，以及磷蛋白-蛋白质表面相互作用的抑制剂测定。
In this Feature Article, we focus on recent advances in our research on molecular recognition and fluorescence sensing of phosphate anion derivatives of biological importance. Because of their significant roles in biological systems, considerable efforts have been devoted to developing detection or determination systems. However, the recognition and sensing of these anion species under aqueous biological conditions using small-molecular chemosensors still remain as a challenging research topic. We have been developing a variety of artificial receptors and fluorescent chemosensors for phosphoproteins and nucleoside polyphosphates in recent years. They consist of a binuclear Zn(II)-dipicolylamine (Dpa) complex as a common binding motif for phosphate anion derivatives. Taking advantage of their strong binding affinities or high sensing abilities, a variety of biological assay systems have also been successfully developed, which includes the enzyme assays such as the kinase, phosphatase and glycosyltransferase reaction, as well as an inhibitor assay for the phosphoprotein-protein surface interaction.