An optimized approach for the assessment of sexual behavior in male rats
An optimized approach for the assessment of sexual behavior in male rats
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DOI：
10.1016/s0890-6238(98)00051-3
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发表时间：
1998-11-01
影响因子：
3.3
通讯作者：
Faqi, AS
中科院分区：
文献类型：
--
作者：
Chahoud, I;Faqi, AS
改进和优化用于检测实验动物生殖障碍的方法是该领域研究人员面临的主要挑战之一。传统的测试雄性性行为的方法是，在测试之前，通过注射苯甲酸雌二醇和黄体酮，切除卵巢使雌性接受性行为，然后在周期的黑暗阶段，在昏暗的红光下，接受雌性与暴露的雄性大鼠交配，然后直接观察交配活动。去卵巢的雌性大鼠对激素注射的反应可能不同(个体差异)，导致前凸强度的差异，此外，通过对交配活动的直接视觉和瞬时评估获得的数据非常主观，可能会产生不准确的结果。在优化的方法中，确定雌性大鼠的性周期，只选择发情期的大鼠与有性经验的雄性大鼠交配。交配活动被录像，从而能够正确地观察和评估交配行为的不同组成部分。这种方法通过避免手术干预来促进动物福利，并使录像带能够作为永久文件保留。(C)1998年爱思唯尔科学公司。
The improvement and optimization of methods used to detect reproductive disorders in experimental animals are among the main challenges facing researchers in this field. The conventional method for testing male sexual behavior uses ovariectomized females rendered sexually receptive by injection of estradiol benzoate and progesterone prior to testing, The receptive females are then mated with exposed male rats during the dark phase of the cycle under dim red light followed by direct visual and momentary observation of the mating activity. The ovariectomized females may respond differently to hormonal injection (individual differences), leading to variations in the intensity of lordosis, Additionally, the data obtained by the direct visual and momentary evaluation of copulatory activity are very subjective and may produce inaccurate results, In the optimized method, the sexual cycles of female rats are determined, and only those in estrus are selected and mated with sexually experienced male rats. The mating activity is videotaped, enabling the correct observation and evaluation of the different components of mating behavior. This method fosters animal welfare by avoiding surgical intervention and enables the videotape to be kept as permanent documentation. (C) 1998 Elsevier Science Inc.