Functional characterization of the BnNCED3 gene in Brassica napus
Functional characterization of the BnNCED3 gene in Brassica napus
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甘蓝型油菜 BnNCED3 基因的功能特征
DOI：
10.1016/j.plantsci.2016.11.012
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发表时间：
2016
期刊：
影响因子：
5.2
通讯作者：
Cai Weiming
中科院分区：
文献类型：
--
作者：
Xu Peipei;Cai Weiming
脱落酸 (ABA) 与植物对各种环境胁迫的适应以及种子休眠、叶片衰老和器官脱落进程的调节有关。 9-顺式环氧类胡萝卜素双加氧酶 (NCED) 家族蛋白对顺式环氧类胡萝卜素的裂解是调节植物脱落酸 (ABA) 合成的关键步骤。本研究从芸苔属植物中分离得到NCED家族基因BnNCED3。 BnNCED3编码592个氨基酸的蛋白质，其氨基酸序列与拟南芥AtNCED3蛋白质具有高度相似性。表达模式测定表明，BnNCED3 在所有检查的器官中以不同水平普遍表达。此外，BnNCED3的过表达有助于转基因拟南芥植物中ABA的积累以及NO和ROS的产生，从而增强非生物胁迫耐受性。这些实验还表明BnNCED3参与转基因拟南芥植物发育的控制，例如抑制种子发芽、侧根萌发、早期阶段变化和增强ABA相关的叶片衰老。总之，这些结果表明 BnNCED3 通过 ABA 生物合成的调节至少部分参与胁迫适应和植物发育。
Abscisic acid (ABA) has been implicated in plant adaptation to various environmental stresses and the regulation of seed dormancy, leaf senescence and organ abscission progression. The cleavage ofcis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (NCED) family proteins is a critical step in the regulation of abscisic acid (ABA) synthesis in plants. In the present study, the NCED family geneBnNCED3was isolated fromBrassicanapus. BnNCED3 encodes a 592-amino acid protein with high amino acid sequence similarity to theArabidopsisAtNCED3 protein. Expression pattern assays revealed thatBnNCED3is ubiquitously expressed at different levels in all the examined organs. Furthermore, the overexpression ofBnNCED3contributed to ABA accumulation and NO and ROS generation in transgenicArabidopsisplants, thereby enhancing abiotic stress tolerance. These experiments also indicated the involvement ofBnNCED3in the control of plant development in transgenicArabidopsis, such as the inhibition of seed germination, lateral root initiation, early phase changes and the enhancement of ABA-associated leaf senescence. Together, these results indicated thatBnNCED3is at least partly involved in both stress adaptation and plant development through the regulation of ABA biosynthesis.