pH-Responsive metal-organic framework encapsulated gold nanoclusters with modulated release to enhance photodynamic therapy/chemotherapy in breast cancer
pH-Responsive metal-organic framework encapsulated gold nanoclusters with modulated release to enhance photodynamic therapy/chemotherapy in breast cancer
复制标题
pH响应金属有机框架封装金纳米团簇并调节释放以增强乳腺癌的光动力疗法/化疗
DOI：
10.1039/c9tb02621e
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发表时间：
2020
影响因子：
7
通讯作者：
Leyong Zeng
中科院分区：
文献类型：
--
作者：
Luyun Zhang;Yang Gao;Sijia Sun;Zihou Li;Aiguo Wu;Leyong Zeng
超小尺寸的金纳米团簇（AuNCs）作为新型无机光敏剂，在光动力治疗（PDT）中已被证明具有广阔的前景，但由于血液循环短，其应用受到限制。因此，开发基于 AuNC 的刺激响应纳米探针以实现高效的 PDT 非常重要。在这里，合成了金属有机框架（MOF，ZIF-8）封装的AuNCs（AuNCs@MOF），然后将其负载阿霉素（DOX）以获得具有调节释放的pH响应纳米探针（AuNCs@MOF-DOX），用于增强PDT/化疗。在酸性肿瘤微环境中，ZIF-8的结构崩溃，加速肿瘤细胞中AuNCs和DOX的释放，增强PDT/化疗的效果。在670 nm激光照射下，产生大量单线态氧，在pH值为5.5时DOX释放率增加至77.1%。通过单一PDT和单一化疗，肿瘤仅被部分抑制，但通过PDT和化疗联合使用，肿瘤完全消失。制备的pH响应型AuNCs@MOF-DOX纳米探针具有调节释放功能，表现出优异的PDT/化疗性能，将成为重要的协同治疗的双功能纳米探针。
Gold nanoclusters (AuNCs) with an ultra-small size, as new inorganic photosensitizers, have been shown to be promising in photodynamic therapy (PDT), but their application has been restricted due to short blood circulation. It is therefore important to develop stimuli-responsive AuNC-based nanoprobes to achieve highly efficient PDT. Here, metal–organic framework (MOF, ZIF-8) encapsulated AuNCs (AuNCs@MOF) were synthesized, and then they were loaded with doxorubicin (DOX) to obtain pH-responsive nanoprobes (AuNCs@MOF-DOX) with modulated release for enhanced PDT/chemotherapy. In an acidic tumor microenvironment, the structure of ZIF-8 collapsed, accelerating the release of the AuNCs and DOX in the tumor cells, and enhancing the performance of PDT/chemotherapy. Under irradiation with a 670 nm laser, a large amount of singlet oxygen was generated, and the release rate of DOX increased to 77.1% at a pH value of 5.5. By single PDT and single chemotherapy, the tumors were only partially inhibited, but they completely disappeared using the combination of PDT and chemotherapy. The prepared pH-responsive AuNCs@MOF-DOX nanoprobes with modulated release showed excellent PDT/chemotherapy performance, and will be important bi-functional nanoprobes for synergistic therapy.