Hypochlorous Acid-Generating Electrochemical Catheter Prototype for Prevention of Intraluminal Infection.
Hypochlorous Acid-Generating Electrochemical Catheter Prototype for Prevention of Intraluminal Infection.
复制标题
用于预防腔内感染的次氯酸生成电化学导管原型。
DOI：
10.1128/spectrum.00557-21
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发表时间：
2021-10-31
影响因子：
3.7
通讯作者：
Patel R
中科院分区：
文献类型：
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作者：
Cano EJ;Flurin L;Mohamed A;Greenwood-Quaintance KE;Raval YS;Beyenal H;Patel R
中心线相关血流感染(CLABSI)会导致死亡率和成本。虽然无菌敷料和抗生素浸渍的导管可以防止一些腔外感染，但腔内感染仍然是CLABSI的一个来源。在这项概念验证研究中，开发了一种能够在铝内使用铂电极电化学产生次氯酸的电化学血管内导管(e-导管)原型，该电极相对于饱和的银/氯化银参比电极以伏特(VAG/AgCl)为单位在恒定电极电位下极化。在1.5VAG/AgCl下预极化24 h后，测试它们对导管相关感染的金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌和大肠埃希菌的活性。E导尿管的HOCl值为15.86 ± 4.03 μM，平均pH值为6.14 ± 0.79。留置导尿管预防了4种细菌的感染，48h时平均下降8.41log10cfu/ml， ± 为0.61log10cfu/ml。应进一步开发持续产生低HOCl量的极化电子导管，以防止腔内感染。重要性与导管相关的感染构成了卫生保健的经济和死亡负担。有几种方法可以降低感染风险，但只有少数几种方法侧重于预防腔内感染，这种感染发生在长期导管中，最常用于透析、延长治疗或化疗。研制了一种名为“e导管”的导管原型，它由三个电极组成，能够在管腔内电化学产生次氯酸(HOCl)。当在1.5V极化时，溶液中的氯离子被氧化以持续产生少量的HOCl，这在导管的管腔中显示出抗菌活性。在这里，这个原型被证明能够在初步的体外导管模型中产生HOCl2并防止感染。这种方法是预防导管感染的一种潜在策略。
Central line-associated bloodstream infection (CLABSI) contributes to mortality and cost. While aseptic dressings and antibiotic-impregnated catheters prevent some extraluminal infections, intraluminal infections remain a source of CLABSIs. In this proof-of-concept study, an electrochemical intravascular catheter (e-catheter) prototype capable of electrochemically generating hypochlorous acid intraluminally using platinum electrodes polarized at a constant potential of 1.5 electrode potential relative to saturated silver/silver chloride reference electrode measured in volts (VAg/AgCl) was developed. After 24 h of prepolarization at 1.5 VAg/AgCl, their activity was tested against clinical isolates of Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, and Escherichia coli derived from catheter-related infections. e-catheters generated a mean HOCl concentration of 15.86 ± 4.03 μM and had a mean pH of 6.14 ± 0.79. E-catheters prevented infections of all four species, with an average reduction of 8.41 ± 0.61 log10 CFU/ml at 48 h compared to controls. Polarized e-catheters which generate low amounts of HOCl continuously should be further developed to prevent intraluminal infection. IMPORTANCE Catheter-related infections constitute an economic and mortality burden in health care. Several options are available to reduce the risk of infection, but only a few focus on preventing intraluminal infection, which occurs in long-term catheters, most often used for dialysis, prolonged treatment, or chemotherapy. A prototype of a catheter called an “e-catheter” composed of three electrodes, capable of producing hypochlorous acid (HOCl) electrochemically in its lumen, was developed. When polarized at 1.5 V, chloride ions in the solution are oxidized to continuously produce low amounts of HOCl, which exhibits antibacterial activity in the lumen of the catheter. Here, this prototype was shown to be able to generate HOCl as well as prevent infection in a preliminary in vitro catheter model. This approach is a potential strategy for catheter infection prevention.