ADENOSINE STIMULATES PROLIFERATION OF HUMAN ENDOTHELIAL-CELLS IN CULTURE
ADENOSINE STIMULATES PROLIFERATION OF HUMAN ENDOTHELIAL-CELLS IN CULTURE
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DOI：
10.1152/ajpheart.1993.265.1.h131
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发表时间：
1993-07-01
影响因子：
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通讯作者：
DOBSON, JG
中科院分区：
文献类型：
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作者：
ETHIER, MF;CHANDER, V;DOBSON, JG
通过在人脐静脉来源的培养物的培养基中加入腺苷来研究腺苷对人内皮细胞增殖的影响。在10 μ M腺苷中生长4-7天的培养物上的细胞计数比对照培养物的计数高41-53%。相反，10 μ M腺苷对人成纤维细胞株（IMR-90）的生长没有影响。无论是肌苷或2 '，5'-双脱氧腺苷影响内皮细胞的生长在0.1或10 μ M的浓度。此外，腺苷脱氨酶废除了添加腺苷的增殖作用，并抑制增殖的16%，在控制文化，表明内源性腺苷可能会提高文化中的增殖。腺苷受体拮抗剂，8-苯基茶碱，在0.1和1.0 μ M阻断10 μ M腺苷引起的增殖增强。添加10 μ M腺苷增强内皮细胞培养物中的DNA合成，如通过增加[H-3]胸苷掺入酸不溶性细胞材料所示。结果表明，除了生理浓度的腺苷人脐静脉内皮细胞培养物刺激增殖，可能通过表面受体，并表明，腺苷可能是一个因素，人内皮细胞生长和可能的血管生成。
The effect of adenosine on proliferation of human endothelial cells was investigated by adding adenosine to the medium of cultures derived from human umbilical veins. Cell counts on cultures grown in 10 muM adenosine for 4-7 days were 41-53% greater than counts from control cultures. In contrast, 10 muM adenosine had no effect on growth of a human fibroblast cell strain (IMR-90). Neither inosine nor 2',5'-dideoxyadenosine influenced endothelial cell growth at concentrations of 0.1 or 10 muM. Addition of adenosine deaminase abolished the proliferative effect of added adenosine and inhibited proliferation by 16% in control cultures, suggesting that endogenous adenosine may enhance proliferation in culture. The adenosine receptor antagonist, 8-phenyltheophylline, at 0.1 and 1.0 muM blocked the enhanced proliferation caused by 10 muM adenosine. Addition of 10 muM adenosine enhanced DNA synthesis in endothelial cell cultures as indicated by an increased incorporation of [H-3]thymidine into acid-insoluble cell material. The results indicate that addition of physiological concentrations of adenosine to human umbilical vein endothelial cell cultures stimulates proliferation, possibly via a surface receptor, and suggest that adenosine may be a factor for human endothelial cell growth and possibly angiogenesis.