Magnetically driven active topography for long-term biofilm control
Magnetically driven active topography for long-term biofilm control
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DOI：
10.1038/s41467-020-16055-5
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发表时间：
2020-05-05
影响因子：
16.6
通讯作者：
Ren, Dacheng
中科院分区：
文献类型：
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作者：
Gu, Huan;Lee, Sang Won;Ren, Dacheng
留置医疗器械上微生物生物膜的形成会导致持续性感染，而这些感染不能用传统的抗生素治愈。为了解决这一未解决的挑战，我们设计了具有微米大小的柱子的可调主动表面形貌，可以在电磁场中以可编程的频率和力水平跳动。与平坦和静态对照相比，优化设计的活性地形防止了生物被膜的形成，并去除了已建立的泌尿系致病大肠埃希菌(UPEC)、铜绿假单胞菌和金黄色葡萄球菌的生物被膜，生物量减少高达3.7log。此外，分离的生物膜细胞被发现对杀菌抗生素的敏化程度与指数期浮游细胞相当。基于这些发现，设计了一个原型导尿管，并发现在人工尿液流动下至少保持清洁30天，而对照导尿管在5天内被UPEC生物膜堵塞。生物膜的形成是留置医疗器械的主要问题。在这里，作者报告了一种磁性响应型微柱表面的开发，用于受控地预防和去除生物膜，这也增加了对抗生素的敏感性。
Microbial biofilm formation on indwelling medical devices causes persistent infections that cannot be cured with conventional antibiotics. To address this unmet challenge, we engineer tunable active surface topographies with micron-sized pillars that can beat at a programmable frequency and force level in an electromagnetic field. Compared to the flat and static controls, active topographies with the optimized design prevent biofilm formation and remove established biofilms of uropathogenic Escherichia coli (UPEC), Pseudomonas aeruginosa, and Staphylococcus aureus, with up to 3.7 logs of biomass reduction. In addition, the detached biofilm cells are found sensitized to bactericidal antibiotics to the level comparable to exponential-phase planktonic cells. Based on these findings, a prototype catheter is engineered and found to remain clean for at least 30 days under the flow of artificial urine medium, while the control catheters are blocked by UPEC biofilms within 5 days. Biofilm formation is a major problem in indwelling medical devices. Here, the authors report on the development of a magnetically responsive micro pillar surface for the controlled prevention and removal of biofilms which also increased sensitivity to antibiotics.