EFFECT OF VARIOUS PROCEDURES ON THE VIABILITY OF MOUSE EMBRYOS CONTAINING HALF THE NORMAL NUMBER OF BLASTOMERES
EFFECT OF VARIOUS PROCEDURES ON THE VIABILITY OF MOUSE EMBRYOS CONTAINING HALF THE NORMAL NUMBER OF BLASTOMERES
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DOI：
10.1530/jrf.0.0690315
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
MCLAREN, A
中科院分区：
文献类型：
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作者：
TSUNODA, Y;MCLAREN, A
从8细胞期或致密期产生的半个胚胎在体外培养1-2天，并在假孕的不同阶段移植到受体的输卵管或子宫中。活胎率低（8-12%），但有1组（27%）的一半胚胎在体外培养1天，并在妊娠第1天移植到输卵管中。然而，即使在该组中，单卵双胞胎的产生率也很低（10个中有1个）。在妊娠第18天，移植半个胚胎后的胎仔体重显著低于移植相似处理但未分割的胚胎后的胎仔体重。将从2细胞期产生的半个胚胎插入空卵带中，包埋在琼脂中，在结扎的小鼠输卵管中培养2-4天，并在妊娠或假孕的第1天转移到受体雌性的输卵管中。将培养2- 3d的双胚与对照胚胎一起移植到假孕受体，在妊娠18-19 d时，获得4对单卵双胎和5对单胎，存活率为65%。
Half embryos produced from 8 cell or compacted stages were cultured in vitro for 1-2 days and transferred to oviducts or uteri of recipients at different stages of pseudopregnancy. The proportion of live fetuses was low (8-12%), except for 1 group (27%) in which half embryos were cultured in vitro for 1 day and transferred into oviducts on the 1st day of pregnancy. Monozygotic twin production rate, however, was low (1 of 10) even in this group. Fetal weight on day 18 of gestation was significantly lower after transfer of half embryos than after transfer of similarly treated but undivided embryos. Half embryos produced from the 2 cell stage were inserted into empty zonae, embedded in agar, cultured in ligated mouse oviducts for 2-4 days and transferred to oviducts of recipient females on the 1st day of pregnancy or pseudopregnancy. When twin embryos cultured for 2-3 days were transferred to pseudopregnant recipients together with control embryos, 4 sets of monozygotic twins and 5 singletons of 10 sets of twin embryos were obtained on days 18-19 of gestation, giving a survival rate of 65%.