Antioxidant activity of Astragalus polysaccharides and antitumour activity of the polysaccharides and siRNA
Antioxidant activity of Astragalus polysaccharides and antitumour activity of the polysaccharides and siRNA
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黄芪多糖的抗氧化活性及多糖和siRNA的抗肿瘤活性
DOI：
10.1016/j.carbpol.2010.02.048
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发表时间：
2010-09-05
影响因子：
11.2
通讯作者：
Zhang Xue-guang
中科院分区：
文献类型：
--
作者：
Li Rui;Chen Wei-chang;Zhang Xue-guang
采用高效液相色谱法（HPLC）和傅立叶变换红外光谱法（FT-IR）对黄芪多糖进行了分析。通过与六种标准物质的HPLC保留时间比较，发现多糖中含有葡萄糖。在1639和1538 cm（-1）处的吸收峰是羧基C-O键的伸缩振动。在1035和956 cm（-1）处的吸收带表明，萃取物的结构中含有芘单体。通过各种抗氧化试验评价黄芪多糖的抗氧化活性。黄芪多糖具有较强的抗氧化活性。黄芪多糖的体外抗氧化能力与其含量显著相关。除了多糖的抗氧化活性外，它还显示出很强的抗肿瘤活性。然后，将CD 40基因siRNA质粒（Psilencer 1. 0-U6-PI3K-siRNA）。评价siRNA的抗肿瘤活性。在这些样品中，黄芪多糖比siRNA表现出更强的抗肿瘤效力。黄芪多糖和siRNA的抗肿瘤作用机制可能与降低细胞内CD 40有关。(C)2010爱思唯尔有限公司版权所有。
In this study, Astragalus polysaccharides was analysed using high performance liquid chromatography (HPLC) and Fourier transform infrared (FT-IR). Compared with retention time of six standard compounds in HPLC analyses, we found that the polysaccharides were composed of glucose. The absorption bands at 1639 and 1538 cm(-1) were attributed to the stretching vibration of the C-O bond of carboxyl group. The absorption bands at 1035 and 956 cm(-1) suggested that the extract contained pyrene monomer in its structure. The antioxidant activity of Astragalus polysaccharides was evaluated by various antioxidant assays. Astragalus polysaccharides showed strong antioxidant activity in all the tested methods. The in vitro antioxidant capacity of Astragalus polysaccharides was significantly correlated with its content. In addition to the antioxidant activity of the polysaccharides, it still showed strong antitumour activity. Then, CD40 gene siRNA plasmid (Psilencer1. 0-U6-P I3K-siRNA) was constructed. Antitumour activity of si RNA was evaluated. Among these samples, Astragalus polysaccharides exhibited more antitumour potency than siRNA. Mechanism of antitumour activity of Astragalus polysaccharides and si RNA may be explained by decreasing CD40 in cells. (C) 2010 Elsevier Ltd. All rights reserved.