Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome
Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome
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DOI：
10.1016/s0888-7543(03)00004-1
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发表时间：
2003-05-01
期刊：
影响因子：
4.4
通讯作者：
Gerstein, M
中科院分区：
文献类型：
--
作者：
Zhang, ZL;Gerstein, M
人类（核）基因组编码至少79种线粒体核糖体蛋白（MRP），这些蛋白被输入线粒体。使用综合方法，我们发现其中41个在基因组中产生120个假基因。大多数MRP假基因是加工来源的，并且可以比对以匹配功能性MRP mRNA的整个编码区。一个加工的假基因被发现起源于选择性剪接的mRNA转录本。我们还发现了两个重复的假基因，在细胞中转录，通过筛选人类EST数据库证实。我们观察到加工的假基因的数量和基因CD长度之间的显著相关性（R = -0.40; p < 0.001），即，相对较短的基因倾向于具有更多加工的假基因。加工假基因的数量和基因CDS GC含量之间也存在较弱的相关性。我们的研究提供了一个人类MRP假基因的目录，这将是有用的功能MRP基因的研究。它还提供了这些基因进化的分子记录。更多详情请访问http://pseudogene.org/。(C)2003 Elsevier Science（美国）。All rights reserved.
The human (nuclear) genome encodes at least 79 mitochondrial ribosomal proteins (MRPs), which are imported into the mitochondria. Using a comprehensive approach, we find 41 of these give rise to 120 pseudogenes in the genome. The majority of the MRP pseudogenes are of processed origin and can be aligned to match the entire coding region of the functional MRP mRNAs. One processed pseudogene was found to have originated from an alternatively spliced mRNA transcript. We also found two duplicated pseudogenes that are transcribed in the cell as confirmed by screening the human EST database. We observed a significant correlation between the number of processed pseudogenes and the gene CDs length (R = -0.40; p < 0.001), i.e., the relatively shorter genes tend to have more processed pseudogenes. There is also a weaker correlation between the number of processed pseudogenes and the gene CDS GC content. Our study provides a catalogue of human MRP pseudogenes, which will be useful in the study of functional MRP genes. It also provides a molecular record of the evolution of these genes. More details are available at http://pseudogene.org/. (C) 2003 Elsevier Science (USA). All rights reserved.