Insights into Campylobacter jejuni colonization and enteritis using a novel infant rabbit model.
Insights into Campylobacter jejuni colonization and enteritis using a novel infant rabbit model.
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使用新型婴儿兔模型深入了解空肠弯曲菌定植和肠炎
DOI：
10.1038/srep28737
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发表时间：
2016-06-30
影响因子：
4.6
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Shang Y;Ren F;Song Z;Li Q;Zhou X;Wang X;Xu Z;Bao G;Wan T;Lei T;Wang N;Jiao XA;Huang J
空肠弯曲菌相关疾病模型的缺乏长期以来一直是研究这种常见肠道病原体的障碍。在这里，我们使用一只幼兔来研究空肠弯曲菌感染，这使我们能够定义该生物体的几个以前未知但关键的特征。空肠弯曲杆菌能够侵入兔子的全身，并出现类似于许多人类弯曲杆菌病中观察到的腹泻症状。大肠是最一致的定植部位并产生肠道炎症，并诱导特定的细胞因子。筛选了空肠弯曲菌感染期间优先表达的基因，acs、cj1385、cj0259似乎与空肠弯曲菌入侵有关。我们的结果表明，幼兔可以作为研究腹泻弯曲杆菌的替代实验模型，并将有助于探索其他相关病原体的发病机制。
A lack of relevant disease models for Campylobacter jejuni has long been an obstacle to research into this common enteric pathogen. Here we used an infant rabbit to study C. jejuni infection, which enables us to define several previously unknown but key features of the organism. C. jejuni is capable of systemic invasion in the rabbit, and developed a diarrhea symptom that mimicked that observed in many human campylobacteriosis. The large intestine was the most consistently colonized site and produced intestinal inflammation, where specific cytokines were induced. Genes preferentially expressed during C. jejuni infection were screened, and acs, cj1385, cj0259 seem to be responsible for C. jejuni invasion. Our results demonstrates that the infant rabbit can be used as an alternative experimental model for the study of diarrheagenic Campylobacter species and will be useful in exploring the pathogenesis of other related pathogens.