Osteoblasts proliferation and differentiation stimulating activities of the main components of Fructus Psoraleae corylifoliae
Osteoblasts proliferation and differentiation stimulating activities of the main components of Fructus Psoraleae corylifoliae
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DOI：
10.1016/j.phymed.2013.09.015
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发表时间：
2014-03-15
期刊：
影响因子：
7.9
通讯作者：
Chen, Zhi P.
中科院分区：
文献类型：
--
作者：
Li, Wei D.;Yan, Cui P.;Chen, Zhi P.
骨质疏松症是一种导致骨折风险增加的骨骼疾病。补骨脂（黄豆果）在中国被广泛用于治疗骨折和关节疾病已有数千年的历史。这项研究的目的是筛选可能具有刺激成骨细胞增殖和分化的黄豆果实的活性成分。建立了高效液相色谱法分析黄豆果实中的主要成分。通过与相应标准物质的保留时间比较，共鉴定出 11 种化合物。采用MU和ALP方法测定成骨细胞增殖和分化活性。淫羊藿苷（一种异戊二烯化黄酮苷）作为阳性对照。 Bavachin 和 isobavachin 显着刺激细胞增殖，而补骨脂酚则表现出更强的促进成骨细胞分化的作用。所有这些化合物都具有特征结构，在它们的每个分子主链上都连接有异戊二烯化侧链。这些结果导致一个假设：异戊二烯基可能对于表现出该活性至关重要。这些化合物与小鼠原代颅骨成骨细胞中异戊烯基的构效关系需要进一步研究。 (C) 2013 爱思唯尔有限公司。版权所有。
Osteoporosis is a disease of bones that leads to an increased risk of fracture. Fructus of Psoralea corylifolia L. (scurfpea fruit) is commonly utilized for treating bone fractures and joint diseases for thousands of years in China. This study was aimed to screen active principles, which might have the potency to stimulate osteoblasts proliferation and differentiation from scurfpea fruit. A HPLC method was established to analyze the main components in scurfpea fruit. Totally 11 compounds have been identified by comparing their retention time with correspondent standard substances. The MU and ALP methods were utilized for the assay of osteoblasts proliferation and differentiation activity. Icariin, a prenylated flavonoid glycoside was treated as the positive control. Bavachin and isobavachin significantly stimulated cell proliferation, while bakuchiol exhibited stronger effect to enhance osteoblasts differentiation. All these compounds were found with a characterized structure that in each of their molecule backbones, a prenylated side chain was attached. These results lead to a hypothesis that prenyl group might be crucial to exhibit the activity. The structure-effect relationship of these compounds with prenyl group in mouse primary calvarial osteoblasts needs to be explored in further research. (C) 2013 Elsevier GmbH. All rights reserved.