CHROMOSOMAL LOCALIZATION OF 5 MURINE HSP70 GENE FAMILY MEMBERS - HSP70-1, HSP70-2, HSP70-3, HSC70T, AND GRP78
CHROMOSOMAL LOCALIZATION OF 5 MURINE HSP70 GENE FAMILY MEMBERS - HSP70-1, HSP70-2, HSP70-3, HSC70T, AND GRP78
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DOI：
10.1006/geno.1993.1158
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发表时间：
1993-04-01
期刊：
影响因子：
4.4
通讯作者：
KOZAK, CA
中科院分区：
文献类型：
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作者：
HUNT, CR;GASSER, DL;KOZAK, CA
70，000-D热休克蛋白（HSP 70）基因家族包括热诱导和组成型表达基因。我们已经将五个小鼠HSP 70基因定位到三条不同染色体上的特定位点。利用Southern杂交技术对中国仓鼠X小鼠体细胞DNA进行分析，将78,000-D葡萄糖调节蛋白（Grp 78）基因定位于2号染色体，将骡生殖细胞特异性Hsp 70 - 2和Hsc 70 t基因分别定位于12号染色体和17号染色体，将热诱导性Hsp 70 - 3基因也定位于17号染色体。Southern杂交分析证实了Grp 78位于Chr 2上，其顺序为：着丝粒-Vim-Vim-Grp 78-Hc。类似的分析也证实了Hsp 70 - 2的初始定位为Chr 12，顺序为：Hsp 70 -2-Aat-Igh。通过Southern杂交分析，进一步将Chr 17上的Hsp 70 - 3和Hsc 70 t-Bat-6基因沿着热诱导的Hsp 70 - 1基因定位于H-2组织相容性区域，顺序为：Hsp 70 -1-Hsp 70 -3-Hsc 70 t-Bat-6（人G7 a，缬氨酰-tRNA合成酶）。
The 70,000-D heat shock protein (HSP70) gene family includes both heat-inducible and constitutively expressed genes. We have mapped five murine HSP70 genes to specific sites on three separate chromosomes. Southern blot analysis of Chinese hamster X mouse somatic cell DNAs was used to assign the gene for the 78,000-D glucose-regulated protein (Grp78) to Chromosome (Chr) 2, the mule germ cell-specificHsp70-2andHsc70tgenes to Chr 12 and Chr 17, respectively, and the heat-inducibleHsp70-3gene also to Chr 17. Southern blot analysis of DNA from the progeny of two multilocus crosses confirmed theGrp78location on Chr 2 and suggested the order: centromere-Vim-Abl-Grp78-Hc. Similar analysis also confirmed the initialHsp70-2assignment to Chr 12 with the order:Hsp70-2-Aat-Igh. TheHsp70-3andHsc70tgenes on Chr 17, along with the heat-inducibleHsp70-1gene, were further localized by Southern blot analysis of genomic clones to theH-2histocompatibility region with the order:Hsp70-1-Hsp70-3-Hsc70t-Bat-6(human G7a, valyl-tRNA synthetase).