NT-3, BDNF, AND NGF IN THE DEVELOPING RAT NERVOUS-SYSTEM - PARALLEL AS WELL AS RECIPROCAL PATTERNS OF EXPRESSION
NT-3, BDNF, AND NGF IN THE DEVELOPING RAT NERVOUS-SYSTEM - PARALLEL AS WELL AS RECIPROCAL PATTERNS OF EXPRESSION
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DOI：
10.1016/0896-6273(90)90089-x
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发表时间：
1990-10-01
期刊：
影响因子：
16.2
通讯作者：
YANCOPOULOS, GD
中科院分区：
文献类型：
--
作者：
MAISONPIERRE, PC;BELLUSCIO, L;YANCOPOULOS, GD
为了深入了解神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养素3（NT-31）在体内作用的部位和阶段特异性，我们比较了发育和成年大鼠中这三种相关神经营养因子以及NGF受体的基因表达模式。这些相关的神经营养因子的初始胚胎表达与神经发生的开始大致一致。然而，这三种因子在此时和整个发育中的神经系统中表达的水平是截然不同的。NT-3是迄今为止在CNS的未成熟区域中表达最高的，其中神经元前体的增殖、迁移和分化正在进行中。NT-3表达随着这些区域的成熟而显著降低。相比之下，BDNF的表达在CNS的发育区域中是低的，并且随着这些区域的成熟而增加。NCF的表达在不同CNS区域的发育过程中有所不同，但与NT-3和BDNF相比并不一致。尽管存在巨大的差异，但NT-3、BDNF和NGF确实有一个惊人的相似之处--在成年海马中的高水平表达。我们的观察结果与NT-3、BDNF和NGF在体内具有平行和相互作用的观点一致。
To obtain insight into the site and stage specificity of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin3(NT-31 action in vivo, we compared the expression patterns of the genes for these three related neurotrophic factors as well as for the NGF receptor in developing and adult rats. Initial embryonic expression of these related neurotrophic factors approximately coincides with the onset of neurogenesis. However, the levels at which the three factors are expressed at this time and throughout the developing nervous system are dramatically different. NT-3 is by far the most highly expressed in immature regions of the CNS in which proliferation, migration, and differentiation of neuronal precursors is ongoing. NT-3 expression dramatically decreases with maturation of these regions. By contrast, BDNF expression is low in developing regions of the CNS and increases as these regions mature. NCF expression varies during the development of discrete CNS regions, but not in any consistent manner compared with NT-3 and BDNF. Despite the dramatic variations, NT-3, BDNF, and NGF do share one striking similarity- high level expression in the adult hippocampus. Our observations are consistent with the idea that NT-3, BDNF, and NGF have parallel aswell as reciprocal roles in vivo.