ROLE OF THE ACCESSORY GENE REGULATOR (AGR) IN PATHOGENESIS OF STAPHYLOCOCCAL OSTEOMYELITIS
ROLE OF THE ACCESSORY GENE REGULATOR (AGR) IN PATHOGENESIS OF STAPHYLOCOCCAL OSTEOMYELITIS
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DOI：
10.1128/iai.63.9.3373-3380.1995
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发表时间：
1995-09-01
影响因子：
3.1
通讯作者：
SMELTZER, MS
中科院分区：
文献类型：
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作者：
GILLASPY, AF;HICKMON, SG;SMELTZER, MS
为了研究辅助基因调节因子（agr）在葡萄球菌骨髓炎中的作用，我们比较了金黄色葡萄球菌骨髓炎分离株（UAMS-1）与携带灭活agr位点的同一菌株（UAMS-4）的衍生物。用兔急性外源性骨髓炎模型评估毒力。通过显微注射将细菌输送到前肢的桡动脉中部区域。4周后，在13只感染大于或等于2 × 10（6）CFU的兔子中有12只的骨中鉴定出UAMS-1，在6只感染小于或等于2 × 10（5）CFU的兔子中有5只的骨中鉴定出UAMS-1。相比之下，UAMS-4在高剂量感染的13例中的6例和低剂量感染的6例中的1例中被发现。此外，基于评估疾病的放射学证据的五点量表，感染UAMS-1的家兔平均得分为2.64 ± 0.30（高剂量）和1.43 +/- 0.39（低剂量），而感染UAMS I的兔的平均评分为0.95 +/- 0.23（高剂量）和0.63 +/- 0.20未感染对照的平均得分为0.53 +/-0.08。在两种剂量下，用UAMS-1获得的结果与用UAMS-4获得的结果显著不同（P小于或等于0.047）。用UAMS-4获得的结果与用任一剂量的UAMS 4的对照获得的结果没有显著差异（P大于或等于0.150）。基于评估疾病的组织病理学证据的类似五点量表，感染UAMS-1的兔的平均得分为2.31 +/- 0.22（高剂量）和1.96 +/- 0.36（低剂量），而感染UAMS-4的兔的平均得分为1.58 +/- 0.29（高剂量），酸0.83 +/- 0.32（低剂量）。对照组的平均得分为0.33 +/- 0.05。在两种剂量下，使用UAMS-1获得的结果与使用UAMS-4获得的结果显著不同（P小于或等于0.040）。然而，用UAMS-4获得的结果仅在高剂量下与对照组有显著差异（P = 0.025）。我们得出结论，agr突变降低了疾病的发生率和严重程度，但也没有消除骨定殖能力和引起骨髓炎的组织病理学证据。
To examine the role of the accessory gene regulator (agr) in staphylococcal osteomyelitis, we compared a Staphylococcus aureus osteomyelitis isolate (UAMS-1) with a derivative of the same strain (UAMS-4) carrying an inactivated agr locus. Virulence was assessed with a rabbit model of acute, exogenous osteomyelitis. bacteria were delivered by microinjection into the midradial region of the forelimb. After 4 weeks, UAMS-1 was identified in the bone of 12 of 13 rabbits infected with greater than or equal to 2 x 10(6) CFU and 5 of 6 infected with less than or equal to 2 x 10(5) CFU. In contrast, UAMS-4 was found in 6 of 13 infected with the higher dose and 1 of 6 infected with the lower dose, Additionally, on the basis of a five-point scale assessing radiographic evidence of disease, rabbits infected with UAMS-1 had average scores of 2.64 +/- 0.30 (high dose) and 1.43 +/- 0.39 (low dose) while rabbits infected with UAMS I had average scores of 0.95 +/- 0.23 (high dose) and 0.63 +/- 0.20 (low dose), Uninfected controls had an average score of 0.53 +/- 0.08, The results obtained with UAMS-1 were significantly different from those obtained with UAMS-4 at both doses (P less than or equal to 0.047). The results obtained with UAMS-4 were not significantly different from those obtained with the controls at either dose of UAMS4 (P greater than or equal to 0.150). On the basis of a similar five-point scale assessing histopathological evidence of disease, rabbits infected with UAMS-1 had average scores of 2.31 +/- 0.22 (high dose) and 1.96 +/- 0.36 (low dose) while rabbits infected with UAMS-4 had average scores of 1.58 +/- 0.29 (high dose) acid 0.83 +/- 0.32 (low dose). Controls had an average score of 0.33 +/- 0.05. The results obtained with UAMS-1 were significantly different from those obtained with UAMS-4 at both doses (P less than or equal to 0.040). However, the results obtained with UAMS-4 were significantly different from the controls only at the high dose of UAMS 4 (P = 0.025), We conclude that mutation of agr reduces the incidence and severity of disease but does nor eliminate the ability to colonize bone and cause histopathological evidence of osteomyelitis.