Evolutionary and expression analysis of miR-#-5p and miR-#-3p at the miRNAs/isomiRs levels.
Evolutionary and expression analysis of miR-#-5p and miR-#-3p at the miRNAs/isomiRs levels.
复制标题
miR--5p 和 miR--3p 在 miRNA/isomiR 水平上的进化和表达分析
DOI：
10.1155/2015/168358
复制
发表时间：
2015
影响因子：
--
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Guo L;Yu J;Yu H;Zhao Y;Chen S;Xu C;Chen F
我们主要从以下三个方面对miR-#-5 p和miR-#-3 p进行了讨论：（1）人类miRNA的初步进化分析;（2）10种典型脊椎动物中不同臂的miRNA的进化分析;（3）利用公开的小RNA测序数据集在miRNA/isomiR水平上分析miRNA的表达模式。我们发现在5 p-miRNA和3 p-miRNA的数量之间没有检测到偏差，而来自miR-#-5 p和miR-#-3 p的miRNA显示可变的核苷酸组成。来自两个臂的isomiR表达谱总是稳定的，但患病样品中的isomiR表达倾向于显示较大程度的分散。miR-#-5 p和miR-#-3 p具有相对独立的进化/表达模式和靶mRNA数据集，这也可能有助于臂选择和/或臂转换现象。同时，miRNA/isomiR表达谱可以通过臂选择和/或臂转换来调节，并且动态miRNA组和isomiR将适应功能和/或进化压力。因此，在miRNA/isomiR水平上进行全面的分析和进一步的实验研究是非常必要的。
We mainly discussed miR-#-5p and miR-#-3p under three aspects: (1) primary evolutionary analysis of human miRNAs; (2) evolutionary analysis of miRNAs from different arms across the typical 10 vertebrates; (3) expression pattern analysis of miRNAs at the miRNA/isomiR levels using public small RNA sequencing datasets. We found that no bias can be detected between the numbers of 5p-miRNA and 3p-miRNA, while miRNAs from miR-#-5p and miR-#-3p show variable nucleotide compositions. IsomiR expression profiles from the two arms are always stable, but isomiR expressions in diseased samples are prone to show larger degree of dispersion. miR-#-5p and miR-#-3p have relative independent evolution/expression patterns and datasets of target mRNAs, which might also contribute to the phenomena of arm selection and/or arm switching. Simultaneously, miRNA/isomiR expression profiles may be regulated via arm selection and/or arm switching, and the dynamic miRNAome and isomiRome will adapt to functional and/or evolutionary pressures. A comprehensive analysis and further experimental study at the miRNA/isomiR levels are quite necessary for miRNA study.