Effects of NSUN2 deficiency on the mRNA 5-methylcytosine modification and gene expression profile in HEK293 cells
Effects of NSUN2 deficiency on the mRNA 5-methylcytosine modification and gene expression profile in HEK293 cells
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NSUN2 缺陷对 HEK293 细胞 mRNA 5-甲基胞嘧啶修饰和基因表达谱的影响
DOI：
10.2217/epi-2018-0169
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发表时间：
2019-03-01
期刊：
影响因子：
3.8
通讯作者：
Cui, Hengmi
中科院分区：
文献类型：
--
作者：
Sun, Zhen;Xue, Songlei;Cui, Hengmi
目的：研究NSUN 2在调控基因表达和细胞增殖中的生物学功能。材料与方法：通过CRISPR/Cas9系统在HEK 293细胞中敲低NSUN 2基因。使用RNA-BisSeq和RNA-Seq评估mRNA m5 C修饰和基因表达。结果：NSUN 2缺陷可抑制HEK 293细胞的增殖和迁移。共鉴定出1185个差异甲基化基因和790个差异表达基因。生物信息学分析表明，差异甲基化基因主要参与调控基因表达。一些与细胞增殖相关的途径被差异表达的基因显著富集。此外，GRB 2和CD 44可能是NSUN 2介导的细胞增殖的关键调节因子。结论：这些发现有助于阐明NSUN 2影响细胞增殖、迁移和其他细胞表型的分子机制。
Aim: To study the biological function of NSUN2 in regulating gene expression and cell proliferation. Materials & methods: The NSUN2 gene was knocked down in HEK293 cells via CRISPR/Cas9 system. mRNA m5C modification and gene expression were assessed using RNA-BisSeq and RNA-Seq. Results: NSUN2 deficiency could inhibit proliferation and migration of HEK293 cells. A total of 1185 differentially methylated genes and 790 differentially expressed genes were identified. Bioinformatics analysis revealed that the differentially methylated genes were mainly involved in regulating gene expression. Some pathways associated with cell proliferation were significantly enriched by the differentially expressed genes. Additionally, GRB2 and CD44 may be key regulators in NSUN2-mediated cell proliferation. Conclusion: These findings help to elucidate the molecular mechanisms by which NSUN2 affects cell proliferation, migration and other cell phenotypes.