ADSORPTION OF BOVINE SERUM-ALBUMIN ONTO SYNTHETIC CALCIUM HYDROXYAPATITE - INFLUENCE OF PARTICLE TEXTURE
ADSORPTION OF BOVINE SERUM-ALBUMIN ONTO SYNTHETIC CALCIUM HYDROXYAPATITE - INFLUENCE OF PARTICLE TEXTURE
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DOI：
10.1016/0927-7765(95)98210-e
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发表时间：
1995-09-25
影响因子：
5.8
通讯作者：
ISHIKAWA, T
中科院分区：
文献类型：
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作者：
KANDORI, K;SHIMIZU, T;ISHIKAWA, T
合成了粒径为27 ~ 103 nm、宽度约为15 nm的羟基磷灰石钙（CaHAPs），研究了在pH 6.0、15 ℃条件下，CaHAPs对牛血清白蛋白（BSA）的吸附行为。合成的CaHAP颗粒的尺寸增加，在老化溶液中的H3 PO 4的浓度增加。BSA在CaHAPs上的吸附等温线为拟Langmuir型。随着CaHAPs颗粒长度的增加，BSA的饱和吸附量（n（s））增加，达到吸附等温线的平台值。然而，没有发现n（s）和CaHAP颗粒中钙磷摩尔比之间的关系。这些结果表明，颗粒的纹理是显着的BSA的吸附。具有平坦表面的大棒状颗粒有利于通过暴露的ac或bc晶面上的大量带正电荷的吸附位点吸附带负电荷的BSA。
Calcium hydroxyapatites (CaHAPs), with particle lengths varying from 27 to 103 nm but similar widths of about 15 nm, were synthesized, and the adsorption behavior of bovine serum albumin (BSA) on these synthetic CaHAPs was investigated at pH 6.0 and 15 degrees C. The size of the synthetic CaHAP particles increased on increasing the concentration of H3PO4 in the aging solution. The adsorption isotherms of BSA on CaHAPs were pseudo-langmuir type. The saturated amounts of adsorbed BSA (n(s)), which an the plateau values of the adsorption isotherms, increased with an increase in particle length of the CaHAPs. However, no relationship was found between n(s) and the molar ratio of calcium to phosphorus in CaHAP particles. These results suggest that the particle texture is significant in the adsorption of BSA. The large rod-like particles with flat surfaces are advantageous for the adsorption of negatively charged BSA by the large number of positively charged adsorption sites on the exposed ac or bc crystal faces.