Aerosolization of cationic lipid-DNA complexes: lipoplex characterization and optimization of aerosol delivery conditions
Aerosolization of cationic lipid-DNA complexes: lipoplex characterization and optimization of aerosol delivery conditions
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DOI：
10.1006/bbrc.2001.5418
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发表时间：
2001-08-24
影响因子：
3.1
通讯作者：
Claude, F
中科院分区：
文献类型：
--
作者：
Guillaume, CR;Delepine, P;Claude, F
本文就基因治疗在肺部疾病治疗中的应用作一综述。它报告了使用超声雾化将质粒-脂质复合物施用于小鼠肺以抑制其上皮细胞。使用超声雾化器将与阳离子脂质复合的质粒雾化。然后，我们表征了lipoplex的大小，并通过电子显微镜观察了lipoplex。最后，我们进一步评估了不同脂质质粒制剂雾化后肺中的体内转基因表达。雾化器产生的颗粒很小，看起来像一个由小的或多或少的立方单位组成的字符串。转基因表达检测在小鼠的肺进一步暴露于气溶胶颗粒产生的超声雾化器20分钟。用我们优化的质粒-脂质-NaCl制剂获得的结果表明，该途径可用于向气道施用适当的基因以治疗呼吸系统疾病。(C)北京：科学出版社.
This study deals with the development of gene therapy in the treatment of lung diseases. It reports on the use of ultrasonic nebulization to administer plasmid-ipid complexes to the lungs of mice to transfect their epithelial cells. A plasmid complexed to cationic lipids was aeosolized using an ultrasonic nebulizer. We then characterized the lipoplex size and visualized the lipoplex by electron microscopy. Finally, we assessed the in vivo transgene expression in the lungs further to the aerosolization of different lipid-plasmid formulations. The nebulizer-generated particles were small and looked like a string composed of little and more or less cubic units. Transgene expression was detected in the lungs of mice further to a 20-min exposure to aerosol particles produced with the ultrasonic nebulizer. The results obtained with our optimized plasmid-ipid-NaCl formulation suggest that this route can be used to administer an appropriate gene to the airways for the treatment of respiratory disorders. (C) 2001 Academic Press.