Separation of insect hemolymph proteins by cascade-mode multi-affinity chromatography.
Separation of insect hemolymph proteins by cascade-mode multi-affinity chromatography.
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通过级联模式多亲和层析分离昆虫血淋巴蛋白。
DOI：
10.1002/arch.940200402
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发表时间：
1992
影响因子：
2.2
通讯作者：
Law,JH
中科院分区：
文献类型：
--
作者：
Samaraweera,P;Porath,J;Law,JH
在含有Zn 2 +-TED、T-gel、Ni 2 +-DPA和苯基琼脂糖凝胶的主线串联柱链和含有Zn 2 +-DPA的侧线柱上，通过级联模式多亲和色谱（CASMAC）对成年雌性天蛾的血淋巴进行分级。该技术分离了一些先前描述的主要血淋巴蛋白，并产生了许多组成简单的组分。这些馏分中的一些只含有较少丰富的蛋白质的Manducahemolymph。因此，CASMAC将是一种非常有用的分离技术，用于纯化和鉴定昆虫血淋巴中的次要蛋白质。© 1992 Wiley利斯公司
The hemolymph of the adult femaleManduca sextawas fractionated by cascade‐mode multiaffinity chromatography (CASMAC) on a main‐line tandem column chain containing Zn2+‐TED, T‐gel, Ni2+‐DPA, and phenylsepharose and a side‐line column containing Zn2+‐DPA. The technique separated some of the previously described major hemolymph proteins, and yielded a number of fractions with simple composition. Some of these fractions contained only less abundant proteins ofManducahemolymph. Thus, it appears that CASMAC would be a very useful fractionation technique for purification and characterization of the minor proteins of insect hemolymph. © 1992 Wiley‐Liss, Inc.